Adaptación de Leptospira interrogans (sensu stricto) al agua dulce.

Gabriel Trueba; Sonia Zapata; Kleber Madrid; Nicolás Peñafiel

Adaptación de Leptospira interrogans (sensu stricto) al agua dulce.

Translated title of the contribution: Adaptation of Leptospira interrograns (sensu stricto) to fresh water

Gabriel Trueba^*
, Sonia Zapata
, Kleber Madrid
, Nicolás Peñafiel

^*Corresponding author for this work

Universidad San Francisco de Quito

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The contact with polluted waters is one of the main risk factors to catch leptospirosis. A study is presented about the adaptation of Leptospira interrograns to nutrient-lacking water media. For this end, leptospires were incubated in distilled water and tampon saline solution for an undetermined period of time. Leptospires kept viable in water for 98 days whereas the incubated ones in tampon saline solution survived 3 weeks only. Protein cellular and external membrane components were analyzed with electrophoresis in acrylamide gel (SDS-PAGE). When OM protein profiles of leptospires kept in water were compared to those OM profiles of cells cultured in ENJA medium, some differences were observed. A 56 kDa protein was present in leptospires kept in water for a week. This protein was identified as GroEL through Western Blot test.

Translated title of the contribution	Adaptation of Leptospira interrograns (sensu stricto) to fresh water
Original language	Spanish
Pages (from-to)	11-14
Number of pages	4
Journal	Revista Cubana de Medicina Tropical
Volume	54
Issue number	1
State	Published - 2002

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Cite this

@article{6ddb25159e6d46479a69e10cfdb8c157,

title = "Adaptaci{\'o}n de Leptospira interrogans (sensu stricto) al agua dulce.",

abstract = "The contact with polluted waters is one of the main risk factors to catch leptospirosis. A study is presented about the adaptation of Leptospira interrograns to nutrient-lacking water media. For this end, leptospires were incubated in distilled water and tampon saline solution for an undetermined period of time. Leptospires kept viable in water for 98 days whereas the incubated ones in tampon saline solution survived 3 weeks only. Protein cellular and external membrane components were analyzed with electrophoresis in acrylamide gel (SDS-PAGE). When OM protein profiles of leptospires kept in water were compared to those OM profiles of cells cultured in ENJA medium, some differences were observed. A 56 kDa protein was present in leptospires kept in water for a week. This protein was identified as GroEL through Western Blot test.",

author = "Gabriel Trueba and Sonia Zapata and Kleber Madrid and Nicol{\'a}s Pe{\~n}afiel",

year = "2002",

language = "Espa{\~n}ol",

volume = "54",

pages = "11--14",

journal = "Revista Cubana de Medicina Tropical",

issn = "0375-0760",

publisher = "Editorial Ciencias Medicas",

number = "1",

}

TY - JOUR

T1 - Adaptación de Leptospira interrogans (sensu stricto) al agua dulce.

AU - Trueba, Gabriel

AU - Zapata, Sonia

AU - Madrid, Kleber

AU - Peñafiel, Nicolás

PY - 2002

Y1 - 2002

N2 - The contact with polluted waters is one of the main risk factors to catch leptospirosis. A study is presented about the adaptation of Leptospira interrograns to nutrient-lacking water media. For this end, leptospires were incubated in distilled water and tampon saline solution for an undetermined period of time. Leptospires kept viable in water for 98 days whereas the incubated ones in tampon saline solution survived 3 weeks only. Protein cellular and external membrane components were analyzed with electrophoresis in acrylamide gel (SDS-PAGE). When OM protein profiles of leptospires kept in water were compared to those OM profiles of cells cultured in ENJA medium, some differences were observed. A 56 kDa protein was present in leptospires kept in water for a week. This protein was identified as GroEL through Western Blot test.

AB - The contact with polluted waters is one of the main risk factors to catch leptospirosis. A study is presented about the adaptation of Leptospira interrograns to nutrient-lacking water media. For this end, leptospires were incubated in distilled water and tampon saline solution for an undetermined period of time. Leptospires kept viable in water for 98 days whereas the incubated ones in tampon saline solution survived 3 weeks only. Protein cellular and external membrane components were analyzed with electrophoresis in acrylamide gel (SDS-PAGE). When OM protein profiles of leptospires kept in water were compared to those OM profiles of cells cultured in ENJA medium, some differences were observed. A 56 kDa protein was present in leptospires kept in water for a week. This protein was identified as GroEL through Western Blot test.

UR - https://www.scopus.com/pages/publications/18344364725

M3 - Artículo

C2 - 15846933

AN - SCOPUS:18344364725

SN - 0375-0760

VL - 54

SP - 11

EP - 14

JO - Revista Cubana de Medicina Tropical

JF - Revista Cubana de Medicina Tropical

IS - 1

ER -

Adaptación de Leptospira interrogans (sensu stricto) al agua dulce.

Abstract

UN SDGs

Other files and links

Fingerprint

Cite this