Anti-inflammatory activities of Italian Chestnut and Eucalyptus honeys on murine RAW 264.7 macrophages

Danila Cianciosi; Tamara Y. Forbes-Hernandez; José M. Alvarez-Suarez; Johura Ansary; Denise Quinzi; Adolfo Amici; María D. Navarro-Hortal; Adelaida Esteban-Muñoz; José Luis Quiles; Maurizio Battino; Francesca Giampieri

doi:10.1016/j.jff.2021.104752

Anti-inflammatory activities of Italian Chestnut and Eucalyptus honeys on murine RAW 264.7 macrophages

Danila Cianciosi
, Tamara Y. Forbes-Hernandez
, José M. Alvarez-Suarez
, Johura Ansary
, Denise Quinzi
, Adolfo Amici
, María D. Navarro-Hortal
, Adelaida Esteban-Muñoz
, José Luis Quiles
, Maurizio Battino^*
, Francesca Giampieri

^*Corresponding author for this work

Universita Politecnica Delle Marche
University of Vigo
King Fahd Medical Research Center
University of Granada
University of Granada
Universidad Europea Del Atlántico (UEA)
Jiangsu University
Faculty of Sciences, King Abdulaziz University

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The aim of this study was to evaluate for the first time the anti-inflammatory effects of chestnut (Castanea sativa) and Eucalyptus (Eucalyptus occidentalis) honeys collected in Italy on murine RAW 264.7 macrophages stressed with the bacterial endotoxin lipopolysaccharide (LPS). Pre-treatment with the two honeys markedly decreased apoptosis and stimulated wound healing in macrophages stressed with LPS, where they also reduced the intracellular levels of ROS and nitrite. Additionally, treatment with LPS decreased the levels of glutathione and suppressed the antioxidant enzyme activities, which were significantly (p < 0.05) increased in the presence of the two honeys, through the modulation of Nrf2 expression; in LPS-stressed macrophages, Chestnut or Eucalyptus honeys protected against inflammation by regulating the main inflammatory biomarkers, including TNF-α, IL-10 and iNOS, through the decrease of NF-кB expression. Finally, they improved mitochondrial respiration and the main related parameters.

Original language	English
Article number	104752
Journal	Journal of Functional Foods
Volume	87
DOIs	https://doi.org/10.1016/j.jff.2021.104752
State	Published - Dec 2021

Keywords

Antioxidant enzymes
Apoptosis
Honey
Inflammation
Mitochondrial respiration
Wound healing

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Cianciosi, D., Forbes-Hernandez, T. Y., Alvarez-Suarez, J. M., Ansary, J., Quinzi, D., Amici, A., Navarro-Hortal, M. D., Esteban-Muñoz, A., Quiles, J. L., Battino, M., & Giampieri, F. (2021). Anti-inflammatory activities of Italian Chestnut and Eucalyptus honeys on murine RAW 264.7 macrophages. Journal of Functional Foods, 87, Article 104752. https://doi.org/10.1016/j.jff.2021.104752

@article{f77de109a99b4a8db76fa7ce84d3d43a,

title = "Anti-inflammatory activities of Italian Chestnut and Eucalyptus honeys on murine RAW 264.7 macrophages",

abstract = "The aim of this study was to evaluate for the first time the anti-inflammatory effects of chestnut (Castanea sativa) and Eucalyptus (Eucalyptus occidentalis) honeys collected in Italy on murine RAW 264.7 macrophages stressed with the bacterial endotoxin lipopolysaccharide (LPS). Pre-treatment with the two honeys markedly decreased apoptosis and stimulated wound healing in macrophages stressed with LPS, where they also reduced the intracellular levels of ROS and nitrite. Additionally, treatment with LPS decreased the levels of glutathione and suppressed the antioxidant enzyme activities, which were significantly (p < 0.05) increased in the presence of the two honeys, through the modulation of Nrf2 expression; in LPS-stressed macrophages, Chestnut or Eucalyptus honeys protected against inflammation by regulating the main inflammatory biomarkers, including TNF-α, IL-10 and iNOS, through the decrease of NF-кB expression. Finally, they improved mitochondrial respiration and the main related parameters.",

keywords = "Antioxidant enzymes, Apoptosis, Honey, Inflammation, Mitochondrial respiration, Wound healing",

author = "Danila Cianciosi and Forbes-Hernandez, \{Tamara Y.\} and Alvarez-Suarez, \{Jos{\'e} M.\} and Johura Ansary and Denise Quinzi and Adolfo Amici and Navarro-Hortal, \{Mar{\'i}a D.\} and Adelaida Esteban-Mu{\~n}oz and Quiles, \{Jos{\'e} Luis\} and Maurizio Battino and Francesca Giampieri",

note = "Publisher Copyright: {\textcopyright} 2021",

year = "2021",

month = dec,

doi = "10.1016/j.jff.2021.104752",

language = "Ingl{\'e}s",

volume = "87",

journal = "Journal of Functional Foods",

issn = "1756-4646",

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T1 - Anti-inflammatory activities of Italian Chestnut and Eucalyptus honeys on murine RAW 264.7 macrophages

AU - Cianciosi, Danila

AU - Forbes-Hernandez, Tamara Y.

AU - Alvarez-Suarez, José M.

AU - Ansary, Johura

AU - Quinzi, Denise

AU - Amici, Adolfo

AU - Navarro-Hortal, María D.

AU - Esteban-Muñoz, Adelaida

AU - Quiles, José Luis

AU - Battino, Maurizio

AU - Giampieri, Francesca

PY - 2021/12

Y1 - 2021/12

N2 - The aim of this study was to evaluate for the first time the anti-inflammatory effects of chestnut (Castanea sativa) and Eucalyptus (Eucalyptus occidentalis) honeys collected in Italy on murine RAW 264.7 macrophages stressed with the bacterial endotoxin lipopolysaccharide (LPS). Pre-treatment with the two honeys markedly decreased apoptosis and stimulated wound healing in macrophages stressed with LPS, where they also reduced the intracellular levels of ROS and nitrite. Additionally, treatment with LPS decreased the levels of glutathione and suppressed the antioxidant enzyme activities, which were significantly (p < 0.05) increased in the presence of the two honeys, through the modulation of Nrf2 expression; in LPS-stressed macrophages, Chestnut or Eucalyptus honeys protected against inflammation by regulating the main inflammatory biomarkers, including TNF-α, IL-10 and iNOS, through the decrease of NF-кB expression. Finally, they improved mitochondrial respiration and the main related parameters.

AB - The aim of this study was to evaluate for the first time the anti-inflammatory effects of chestnut (Castanea sativa) and Eucalyptus (Eucalyptus occidentalis) honeys collected in Italy on murine RAW 264.7 macrophages stressed with the bacterial endotoxin lipopolysaccharide (LPS). Pre-treatment with the two honeys markedly decreased apoptosis and stimulated wound healing in macrophages stressed with LPS, where they also reduced the intracellular levels of ROS and nitrite. Additionally, treatment with LPS decreased the levels of glutathione and suppressed the antioxidant enzyme activities, which were significantly (p < 0.05) increased in the presence of the two honeys, through the modulation of Nrf2 expression; in LPS-stressed macrophages, Chestnut or Eucalyptus honeys protected against inflammation by regulating the main inflammatory biomarkers, including TNF-α, IL-10 and iNOS, through the decrease of NF-кB expression. Finally, they improved mitochondrial respiration and the main related parameters.

KW - Antioxidant enzymes

KW - Apoptosis

KW - Honey

KW - Inflammation

KW - Mitochondrial respiration

KW - Wound healing

UR - https://www.scopus.com/pages/publications/85114909981

U2 - 10.1016/j.jff.2021.104752

DO - 10.1016/j.jff.2021.104752

M3 - Artículo

AN - SCOPUS:85114909981

SN - 1756-4646

VL - 87

JO - Journal of Functional Foods

JF - Journal of Functional Foods

M1 - 104752

ER -

Anti-inflammatory activities of Italian Chestnut and Eucalyptus honeys on murine RAW 264.7 macrophages

Abstract

Keywords

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