Cloning of the pfaP gene of Leptospira borgpetersenii

Gabriel A. Trueba; Carole A. Bolin; Richard L. Zuerner

doi:10.1016/0378-1119(95)00201-G

Cloning of the pfaP gene of Leptospira borgpetersenii

Gabriel A. Trueba^*
, Carole A. Bolin
, Richard L. Zuerner

^*Corresponding author for this work

Agricultural Research Service
Iowa State University

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A λgtll library constructed with Leptospira borgpetersenii DNA was screened with monoclonal antibodies (mAb) recognizing a periplasmic flagella-associated protein. A plaque expressing a fusion protein (λF15) which reacted with the mAb was isolated and the nucleotide sequence analyzed. The deduced amino-acid (aa) sequence indicates that the pfaP gene belongs to a group of bacterial genes whose products share aa sequence and possibly functional homologies with sppA, an Escherichia coli signal peptidase-encoding gene.

Original language	English
Pages (from-to)	133-134
Number of pages	2
Journal	Gene
Volume	160
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(95)00201-G
State	Published - 4 Jul 1995
Externally published	Yes

Keywords

Recombinant DNA
pathogen
periplasmic flagella
signal peptidase
spirochete
sppA

Access to Document

10.1016/0378-1119(95)00201-G

Cite this

@article{2d5c80344a3442689c7d873a0747f260,

title = "Cloning of the pfaP gene of Leptospira borgpetersenii",

abstract = "A λgtll library constructed with Leptospira borgpetersenii DNA was screened with monoclonal antibodies (mAb) recognizing a periplasmic flagella-associated protein. A plaque expressing a fusion protein (λF15) which reacted with the mAb was isolated and the nucleotide sequence analyzed. The deduced amino-acid (aa) sequence indicates that the pfaP gene belongs to a group of bacterial genes whose products share aa sequence and possibly functional homologies with sppA, an Escherichia coli signal peptidase-encoding gene.",

keywords = "Recombinant DNA, pathogen, periplasmic flagella, signal peptidase, spirochete, sppA",

author = "Trueba, \{Gabriel A.\} and Bolin, \{Carole A.\} and Zuerner, \{Richard L.\}",

year = "1995",

month = jul,

day = "4",

doi = "10.1016/0378-1119(95)00201-G",

language = "Ingl{\'e}s",

volume = "160",

pages = "133--134",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Cloning of the pfaP gene of Leptospira borgpetersenii

AU - Trueba, Gabriel A.

AU - Bolin, Carole A.

AU - Zuerner, Richard L.

PY - 1995/7/4

Y1 - 1995/7/4

N2 - A λgtll library constructed with Leptospira borgpetersenii DNA was screened with monoclonal antibodies (mAb) recognizing a periplasmic flagella-associated protein. A plaque expressing a fusion protein (λF15) which reacted with the mAb was isolated and the nucleotide sequence analyzed. The deduced amino-acid (aa) sequence indicates that the pfaP gene belongs to a group of bacterial genes whose products share aa sequence and possibly functional homologies with sppA, an Escherichia coli signal peptidase-encoding gene.

AB - A λgtll library constructed with Leptospira borgpetersenii DNA was screened with monoclonal antibodies (mAb) recognizing a periplasmic flagella-associated protein. A plaque expressing a fusion protein (λF15) which reacted with the mAb was isolated and the nucleotide sequence analyzed. The deduced amino-acid (aa) sequence indicates that the pfaP gene belongs to a group of bacterial genes whose products share aa sequence and possibly functional homologies with sppA, an Escherichia coli signal peptidase-encoding gene.

KW - Recombinant DNA

KW - pathogen

KW - periplasmic flagella

KW - signal peptidase

KW - spirochete

KW - sppA

UR - https://www.scopus.com/pages/publications/0029025237

U2 - 10.1016/0378-1119(95)00201-G

DO - 10.1016/0378-1119(95)00201-G

M3 - Artículo

C2 - 7628708

AN - SCOPUS:0029025237

SN - 0378-1119

VL - 160

SP - 133

EP - 134

JO - Gene

JF - Gene

IS - 1

ER -

Cloning of the pfaP gene of Leptospira borgpetersenii

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this