TY - JOUR
T1 - Cultivo de células madre mesenquimales sobre hidrogeles compuestos de nanopartículas de hidroxiapatita y quitosano foto-entrecruzable
AU - Alvarez-Barreto, J.
AU - Márquez, K.
AU - Gallardo, E.
AU - Moret, J.
AU - Benítez, N.
AU - Meyer, M.
AU - Marsiccobetre, S.
AU - Zujur, D.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Chitosan (QT) is a biopolymer that has been used in widely used in bone tissue engineering applications, demonstrating great potential for that purpose. Therefore, the present study aims to develop an in situ crosslinking hydrogel system, composed of chitosan and hydroxyapatite (HAP). Briefly, chitosan was modified, using carbodiimide chemistry, with lactobionic and azidobenzoic acid to make it soluble at physiological pH and photo-crosslinkable, respectively. The modified chitosan was mixed with HAP, in different proportions, and later exposed to UV light, yielding hydrogels. Mesenchymal stem cells, from rat bone marrow, were seeded onto the hydrogels and cultured for 4, 10 and 16 days, under osteogenic and non-osteogenic conditions. Through cell proliferation and alkaline phosphatase activity assays, and confocal microscopy, it was observed that the majority of formulations supported cell adhesion and proliferation, and a significant early osteogenic differentiation in formulations 70:30 and 50:50, QT:HAP. According to these results, the proposed photo-crosslinking system has potential for tissue engineering applications, and further specific studies are proposed for cell differentiation.
AB - Chitosan (QT) is a biopolymer that has been used in widely used in bone tissue engineering applications, demonstrating great potential for that purpose. Therefore, the present study aims to develop an in situ crosslinking hydrogel system, composed of chitosan and hydroxyapatite (HAP). Briefly, chitosan was modified, using carbodiimide chemistry, with lactobionic and azidobenzoic acid to make it soluble at physiological pH and photo-crosslinkable, respectively. The modified chitosan was mixed with HAP, in different proportions, and later exposed to UV light, yielding hydrogels. Mesenchymal stem cells, from rat bone marrow, were seeded onto the hydrogels and cultured for 4, 10 and 16 days, under osteogenic and non-osteogenic conditions. Through cell proliferation and alkaline phosphatase activity assays, and confocal microscopy, it was observed that the majority of formulations supported cell adhesion and proliferation, and a significant early osteogenic differentiation in formulations 70:30 and 50:50, QT:HAP. According to these results, the proposed photo-crosslinking system has potential for tissue engineering applications, and further specific studies are proposed for cell differentiation.
KW - Hydrogels
KW - Hydroxyapatite
KW - Mesenchymal stem cells chitosan
KW - Photo- crosslinking
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85029785251&partnerID=8YFLogxK
U2 - 10.17488/RMIB.38.3.2
DO - 10.17488/RMIB.38.3.2
M3 - Artículo
AN - SCOPUS:85029785251
SN - 0188-9532
VL - 38
SP - 524
EP - 536
JO - Revista Mexicana de Ingenieria Biomedica
JF - Revista Mexicana de Ingenieria Biomedica
IS - 3
ER -