Detection and genotyping of Listeria monocytogenes in artisanal soft cheeses from Ecuador

Estefania Espinosa-Mata; Lorena Mejía; José E. Villacís; Viviana Alban; Sonia Zapata

doi:10.1016/j.ram.2021.02.013

Detection and genotyping of Listeria monocytogenes in artisanal soft cheeses from Ecuador

Estefania Espinosa-Mata
, Lorena Mejía
, José E. Villacís
, Viviana Alban
, Sonia Zapata^*

^*Corresponding author for this work

Universidad San Francisco de Quito
Centro de Referencia Nacional de Resistencia a los Antimicrobianos Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”
Pontificia Universidad Católica del Ecuador

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Listeria monocytogenes is one of the most important bacteria associated with foodborne diseases, soft cheese being an important L. monocytogenes vehicle. In Ecuador, soft cheese is consumed in 84.3% of urban households. We determined the prevalence of L. monocytogenes and serogroups in 260 fresh artisanal soft cheese samples collected in 18 of 24 Ecuadorian provinces. Listeria spp. detection was carried out by culture-dependent and independent methods; 14.23% of samples were positive for L. monocytogenes. Serogroup IVb was found in 83.78% of the food isolates. Serogroups IIb and IIa were present in 8.11% of our isolates. To our knowledge, this is the first report of L. monocytogenes serogroups associated with food in Ecuador; we also found serogroup similarities between cheese isolates and clinical isolates.

Translated title of the contribution	Detección y genotipado de Listeria monocytogenes en quesos frescos artesanales de Ecuador
Original language	English
Pages (from-to)	53-56
Number of pages	4
Journal	Revista Argentina de Microbiologia
Volume	54
Issue number	1
DOIs	https://doi.org/10.1016/j.ram.2021.02.013
State	Published - 1 Jan 2022

Keywords

Artisanal soft cheese
Foodborne disease
Listeria monocytogenes
Listeriosis
Serogroup

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10.1016/j.ram.2021.02.013

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title = "Detection and genotyping of Listeria monocytogenes in artisanal soft cheeses from Ecuador",

abstract = "Listeria monocytogenes is one of the most important bacteria associated with foodborne diseases, soft cheese being an important L. monocytogenes vehicle. In Ecuador, soft cheese is consumed in 84.3\% of urban households. We determined the prevalence of L. monocytogenes and serogroups in 260 fresh artisanal soft cheese samples collected in 18 of 24 Ecuadorian provinces. Listeria spp. detection was carried out by culture-dependent and independent methods; 14.23\% of samples were positive for L. monocytogenes. Serogroup IVb was found in 83.78\% of the food isolates. Serogroups IIb and IIa were present in 8.11\% of our isolates. To our knowledge, this is the first report of L. monocytogenes serogroups associated with food in Ecuador; we also found serogroup similarities between cheese isolates and clinical isolates.",

keywords = "Artisanal soft cheese, Foodborne disease, Listeria monocytogenes, Listeriosis, Serogroup",

author = "Estefania Espinosa-Mata and Lorena Mej{\'i}a and Villac{\'i}s, \{Jos{\'e} E.\} and Viviana Alban and Sonia Zapata",

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year = "2022",

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doi = "10.1016/j.ram.2021.02.013",

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T1 - Detection and genotyping of Listeria monocytogenes in artisanal soft cheeses from Ecuador

AU - Espinosa-Mata, Estefania

AU - Mejía, Lorena

AU - Villacís, José E.

AU - Alban, Viviana

AU - Zapata, Sonia

PY - 2022/1/1

Y1 - 2022/1/1

N2 - Listeria monocytogenes is one of the most important bacteria associated with foodborne diseases, soft cheese being an important L. monocytogenes vehicle. In Ecuador, soft cheese is consumed in 84.3% of urban households. We determined the prevalence of L. monocytogenes and serogroups in 260 fresh artisanal soft cheese samples collected in 18 of 24 Ecuadorian provinces. Listeria spp. detection was carried out by culture-dependent and independent methods; 14.23% of samples were positive for L. monocytogenes. Serogroup IVb was found in 83.78% of the food isolates. Serogroups IIb and IIa were present in 8.11% of our isolates. To our knowledge, this is the first report of L. monocytogenes serogroups associated with food in Ecuador; we also found serogroup similarities between cheese isolates and clinical isolates.

AB - Listeria monocytogenes is one of the most important bacteria associated with foodborne diseases, soft cheese being an important L. monocytogenes vehicle. In Ecuador, soft cheese is consumed in 84.3% of urban households. We determined the prevalence of L. monocytogenes and serogroups in 260 fresh artisanal soft cheese samples collected in 18 of 24 Ecuadorian provinces. Listeria spp. detection was carried out by culture-dependent and independent methods; 14.23% of samples were positive for L. monocytogenes. Serogroup IVb was found in 83.78% of the food isolates. Serogroups IIb and IIa were present in 8.11% of our isolates. To our knowledge, this is the first report of L. monocytogenes serogroups associated with food in Ecuador; we also found serogroup similarities between cheese isolates and clinical isolates.

KW - Artisanal soft cheese

KW - Foodborne disease

KW - Listeria monocytogenes

KW - Listeriosis

KW - Serogroup

UR - https://www.scopus.com/pages/publications/85106310269

U2 - 10.1016/j.ram.2021.02.013

DO - 10.1016/j.ram.2021.02.013

M3 - Artículo

C2 - 33906777

AN - SCOPUS:85106310269

SN - 0325-7541

VL - 54

SP - 53

EP - 56

JO - Revista Argentina de Microbiologia

JF - Revista Argentina de Microbiologia

IS - 1

ER -

Detection and genotyping of Listeria monocytogenes in artisanal soft cheeses from Ecuador

Abstract

Keywords

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