TY - JOUR
T1 - Distribution of blaCTX-M-gene variants in E. coli from different origins in Ecuador
AU - Valenzuela, Xavier
AU - Hedman, Hayden
AU - Villagomez, Alma
AU - Cardenas, Paul
AU - Eisenberg, Joseph N.S.
AU - Levy, Karen
AU - Zhang, Lixin
AU - Trueba, Gabriel
N1 - Publisher Copyright:
© 2023 The Authors
PY - 2023/12
Y1 - 2023/12
N2 - The increasing abundance of extended spectrum β-lactamase (ESBL) genes in E. coli, and other commensal and pathogenic bacteria, endangers the utility of third or more recent generation cephalosporins, which are major tools for fighting deadly infections. The role of domestic animals in the transmission of ESBL carrying bacteria has been recognized, especially in low- and middle-income countries, however the horizontal gene transfer of these genes is difficult to assess. Here we investigate blaCTX-M gene diversity (and flanking nucleotide sequences) in E. coli from chicken and humans, in an Ecuadorian rural community and from chickens in another location in Ecuador. The blaCTX-M associated sequences in isolates from humans and chickens in the same remote community showed greater similarity than those found in E. coli in a chicken industrial operation 200 km away. Our study may provide evidence of blaCTX-M transfer between chickens and humans in the community.
AB - The increasing abundance of extended spectrum β-lactamase (ESBL) genes in E. coli, and other commensal and pathogenic bacteria, endangers the utility of third or more recent generation cephalosporins, which are major tools for fighting deadly infections. The role of domestic animals in the transmission of ESBL carrying bacteria has been recognized, especially in low- and middle-income countries, however the horizontal gene transfer of these genes is difficult to assess. Here we investigate blaCTX-M gene diversity (and flanking nucleotide sequences) in E. coli from chicken and humans, in an Ecuadorian rural community and from chickens in another location in Ecuador. The blaCTX-M associated sequences in isolates from humans and chickens in the same remote community showed greater similarity than those found in E. coli in a chicken industrial operation 200 km away. Our study may provide evidence of blaCTX-M transfer between chickens and humans in the community.
KW - Antimicrobial resistance
KW - Chickens
KW - E. coli
KW - Ecuador
KW - ISEcp1, spacer sequence
KW - Third-generation cephalosporin resistance (TGCR)
KW - bla
UR - http://www.scopus.com/inward/record.url?scp=85176129298&partnerID=8YFLogxK
U2 - 10.1016/j.medmic.2023.100092
DO - 10.1016/j.medmic.2023.100092
M3 - Artículo
AN - SCOPUS:85176129298
SN - 2590-0978
VL - 18
JO - Medicine in Microecology
JF - Medicine in Microecology
M1 - 100092
ER -