Entamoeba histolytica: Mechanism of decrease of virulence of axenic cultures maintained for prolonged periods

Alfonso Olivos; Espiridión Ramos; Mario Nequiz; Carlos Barba; Eusebio Tello; Guadalupe Castañón; Augusto González; Rubén D. Martínez; Irmgard Montfort; Ruy Pérez-Tamayo

doi:10.1016/j.exppara.2005.03.020

Entamoeba histolytica: Mechanism of decrease of virulence of axenic cultures maintained for prolonged periods

Alfonso Olivos^*
, Espiridión Ramos
, Mario Nequiz
, Carlos Barba
, Eusebio Tello
, Guadalupe Castañón
, Augusto González
, Rubén D. Martínez
, Irmgard Montfort
, Ruy Pérez-Tamayo

^*Corresponding author for this work

Universidad Nacional Autónoma de México
Centro de Investigacion y de Estudios Avanzados del Instituto Politécnico Nacional

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Intraportal injection of non-virulent E. histolytica (derived from prolonged axenic culture of virulent E. histolytica) strain HM1-IMSS in normal hamsters results in no liver lesions and disappearance of the parasites 48-72 h after injection. Viability of non-virulent E. histolytica after 2 h of in vitro incubation in either fresh or decomplemented hamster serum is the same as control virulent E. histolytica (50-90%). In hamsters made leukopenic, or both leukopenic + hypocomplementemic, or hypocomplementemic + sephadex microspheres (to produce focal liver ischemia) intraportally injected non-virulent E. histolytica cause no lesions and disappear after 24 h. In addition, neither hypocomplementemia nor immunosuppression with cyclosporin A prolonged the survival of non-virulent E. histolytica. Methyl prednisolone treatment of hamsters resulted in survival of large numbers of non-virulent E. histolytica in the liver, with little inflammation and minimal tissue damage, for up to 7 days. Inflammatory cells (macrophages) would appear to be chiefly responsible for elimination of non-virulent E. histolytica. Parallel experiments with E. dispar suggest a different mechanism for its non-pathogenicity.

Original language	English
Pages (from-to)	309-312
Number of pages	4
Journal	Experimental Parasitology
Volume	110
Issue number	3 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.exppara.2005.03.020
State	Published - Jul 2005
Externally published	Yes

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SDG 3 Good Health and Well-being

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10.1016/j.exppara.2005.03.020

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@article{b04dedf4ecad4a54b5b08cc3c54d0b6a,

title = "Entamoeba histolytica: Mechanism of decrease of virulence of axenic cultures maintained for prolonged periods",

abstract = "Intraportal injection of non-virulent E. histolytica (derived from prolonged axenic culture of virulent E. histolytica) strain HM1-IMSS in normal hamsters results in no liver lesions and disappearance of the parasites 48-72 h after injection. Viability of non-virulent E. histolytica after 2 h of in vitro incubation in either fresh or decomplemented hamster serum is the same as control virulent E. histolytica (50-90\%). In hamsters made leukopenic, or both leukopenic + hypocomplementemic, or hypocomplementemic + sephadex microspheres (to produce focal liver ischemia) intraportally injected non-virulent E. histolytica cause no lesions and disappear after 24 h. In addition, neither hypocomplementemia nor immunosuppression with cyclosporin A prolonged the survival of non-virulent E. histolytica. Methyl prednisolone treatment of hamsters resulted in survival of large numbers of non-virulent E. histolytica in the liver, with little inflammation and minimal tissue damage, for up to 7 days. Inflammatory cells (macrophages) would appear to be chiefly responsible for elimination of non-virulent E. histolytica. Parallel experiments with E. dispar suggest a different mechanism for its non-pathogenicity.",

author = "Alfonso Olivos and Espiridi{\'o}n Ramos and Mario Nequiz and Carlos Barba and Eusebio Tello and Guadalupe Casta{\~n}{\'o}n and Augusto Gonz{\'a}lez and Mart{\'i}nez, \{Rub{\'e}n D.\} and Irmgard Montfort and Ruy P{\'e}rez-Tamayo",

note = "Funding Information: This work was supported in part by CONACyT Grants 30831-M, DGAPA Grant IN-244202 and 241402. We received excellent technical help from Pedro Balderas, V{\'i}ctor Cornejo and Marco E. Gudi{\~n}o as well as the professional animal care of Ricardo Vargas and Daniel S{\'a}nchez Almaraz.",

year = "2005",

month = jul,

doi = "10.1016/j.exppara.2005.03.020",

language = "Ingl{\'e}s",

volume = "110",

pages = "309--312",

journal = "Experimental Parasitology",

issn = "0014-4894",

publisher = "Academic Press Inc.",

number = "3 SPEC. ISS.",

}

TY - JOUR

T1 - Entamoeba histolytica

T2 - Mechanism of decrease of virulence of axenic cultures maintained for prolonged periods

AU - Olivos, Alfonso

AU - Ramos, Espiridión

AU - Nequiz, Mario

AU - Barba, Carlos

AU - Tello, Eusebio

AU - Castañón, Guadalupe

AU - González, Augusto

AU - Martínez, Rubén D.

AU - Montfort, Irmgard

AU - Pérez-Tamayo, Ruy

N1 - Funding Information: This work was supported in part by CONACyT Grants 30831-M, DGAPA Grant IN-244202 and 241402. We received excellent technical help from Pedro Balderas, Víctor Cornejo and Marco E. Gudiño as well as the professional animal care of Ricardo Vargas and Daniel Sánchez Almaraz.

PY - 2005/7

Y1 - 2005/7

N2 - Intraportal injection of non-virulent E. histolytica (derived from prolonged axenic culture of virulent E. histolytica) strain HM1-IMSS in normal hamsters results in no liver lesions and disappearance of the parasites 48-72 h after injection. Viability of non-virulent E. histolytica after 2 h of in vitro incubation in either fresh or decomplemented hamster serum is the same as control virulent E. histolytica (50-90%). In hamsters made leukopenic, or both leukopenic + hypocomplementemic, or hypocomplementemic + sephadex microspheres (to produce focal liver ischemia) intraportally injected non-virulent E. histolytica cause no lesions and disappear after 24 h. In addition, neither hypocomplementemia nor immunosuppression with cyclosporin A prolonged the survival of non-virulent E. histolytica. Methyl prednisolone treatment of hamsters resulted in survival of large numbers of non-virulent E. histolytica in the liver, with little inflammation and minimal tissue damage, for up to 7 days. Inflammatory cells (macrophages) would appear to be chiefly responsible for elimination of non-virulent E. histolytica. Parallel experiments with E. dispar suggest a different mechanism for its non-pathogenicity.

AB - Intraportal injection of non-virulent E. histolytica (derived from prolonged axenic culture of virulent E. histolytica) strain HM1-IMSS in normal hamsters results in no liver lesions and disappearance of the parasites 48-72 h after injection. Viability of non-virulent E. histolytica after 2 h of in vitro incubation in either fresh or decomplemented hamster serum is the same as control virulent E. histolytica (50-90%). In hamsters made leukopenic, or both leukopenic + hypocomplementemic, or hypocomplementemic + sephadex microspheres (to produce focal liver ischemia) intraportally injected non-virulent E. histolytica cause no lesions and disappear after 24 h. In addition, neither hypocomplementemia nor immunosuppression with cyclosporin A prolonged the survival of non-virulent E. histolytica. Methyl prednisolone treatment of hamsters resulted in survival of large numbers of non-virulent E. histolytica in the liver, with little inflammation and minimal tissue damage, for up to 7 days. Inflammatory cells (macrophages) would appear to be chiefly responsible for elimination of non-virulent E. histolytica. Parallel experiments with E. dispar suggest a different mechanism for its non-pathogenicity.

UR - https://www.scopus.com/pages/publications/20444502898

U2 - 10.1016/j.exppara.2005.03.020

DO - 10.1016/j.exppara.2005.03.020

M3 - Artículo

C2 - 15955329

AN - SCOPUS:20444502898

SN - 0014-4894

VL - 110

SP - 309

EP - 312

JO - Experimental Parasitology

JF - Experimental Parasitology

IS - 3 SPEC. ISS.

ER -

Entamoeba histolytica: Mechanism of decrease of virulence of axenic cultures maintained for prolonged periods

Abstract

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