Insulin-like growth factor (IGF) parameters and tools for efficacy: The IGF-I generation test in children

Ron G. Rosenfeld, Caroline Buckway, Karin Selva, Kathie L. Pratt, Jaime Guevara-Aguirre

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Serum levels of growth hormone (GH)-dependent peptides could provide important and valuable measures of GH sensitivity and, potentially, responsiveness. In normal individuals, serum insulin-like growth factor I (IGF-I) concentrations are dependent on the dose of GH given, with IGF-I responsiveness not decreasing with age. Individuals heterozygous for the E180 GH receptor (GHR) splice mutation have normal IGF-I generation, but those homozygous for the E180 splice mutation have very low basal and stimulated IGF-I concentrations. Similar results are observed for the serum IGF-binding protein 3 (IGFBP-3) response to GH, with a correlation between changes in serum concentrations of IGF-I and changes in IGFBP-3 in normal, heterozygotic, GH-insensitive and GH-deficient participants. In individuals with the E180 splice mutation, IGF-I and IGFBP-3 tests show sensitivity and specificity for detecting GH insensitivity (GHI). In children with idiopathic short stature, it appears that some individuals have selective resistance to GH, with their ability to generate IGF-I more impaired than their ability to generate other GH-dependent peptides. This heterogeneous group may require individualization of GH dosage. IGF generation tests remain the best short-term, in vivo test for classic GHI, although diagnostic tests will undoubtedly require further modification to identify milder pathophysiologic abnormalities.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalHormone Research
Issue numberSUPPL. 1
StatePublished - 2004
Externally publishedYes


  • Children
  • Growth hormone
  • Growth hormone deficiency
  • Igf generation
  • Insulin-like growth factor binding protein
  • Insulin-like growth factor i


Dive into the research topics of 'Insulin-like growth factor (IGF) parameters and tools for efficacy: The IGF-I generation test in children'. Together they form a unique fingerprint.

Cite this