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Isolation, culture, cryopreservation, and identification of bovine, murine, and human spermatogonial stem cells

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

6 Scopus citations

Abstract

Spermatogonial stem cells (SSCs) are the germ cells at the basis of spermatogenesis in adult mammals. SSCs offer many biotechnological possibilities and are fundamental cells in the study of spermatogenesis (Aponte, World J Stem Cells 7:669–680, 2015). This chapter describes detailed procedures for SSC isolation, culture, cryopreservation, and characterization in bovine, murine, and human models.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages151-164
Number of pages14
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2155
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell culture
  • Characterization
  • Cryopreservation
  • Functional test
  • Isolation
  • Spermatogenesis
  • Spermatogonial stem cells
  • Testis

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