Isolation of oropouche virus from febrile patient, Ecuador

Emma L. Wise; Steven T. Pullan; Sully Márquez; Verónica Paz; Juan D. Mosquera; Sonia Zapata; Simon K. Jackson; Gyorgy Fejer; Gabriel Trueba; Christopher H. Logue

doi:10.3201/eid2405.171569

Isolation of oropouche virus from febrile patient, Ecuador

Emma L. Wise^*
, Steven T. Pullan
, Sully Márquez
, Verónica Paz
, Juan D. Mosquera
, Sonia Zapata
, Simon K. Jackson
, Gyorgy Fejer
, Gabriel Trueba
, Christopher H. Logue

^*Corresponding author for this work

UK Health Security Agency
University of Plymouth
Hospital Delfina Torres de Concha
Universidad San Francisco de Quito

Research output: Contribution to journal › Letter › peer-review

28 Scopus citations

Abstract

We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.

Original language	English
Pages (from-to)	935-937
Number of pages	3
Journal	Emerging Infectious Diseases
Volume	24
Issue number	5
DOIs	https://doi.org/10.3201/eid2405.171569
State	Published - May 2018

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SDG 3 Good Health and Well-being

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10.3201/eid2405.171569

Cite this

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abstract = "We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.",

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AU - Wise, Emma L.

AU - Pullan, Steven T.

AU - Márquez, Sully

AU - Paz, Verónica

AU - Mosquera, Juan D.

AU - Zapata, Sonia

AU - Jackson, Simon K.

AU - Fejer, Gyorgy

AU - Trueba, Gabriel

AU - Logue, Christopher H.

PY - 2018/5

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N2 - We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.

AB - We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru.

UR - https://www.scopus.com/pages/publications/85045647979

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JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

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ER -

Isolation of oropouche virus from febrile patient, Ecuador

Abstract

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