Abstract
The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
| Original language | English |
|---|---|
| Article number | 1535750 |
| Journal | Journal of Extracellular Vesicles |
| Volume | 7 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Jan 2018 |
| Externally published | Yes |
Keywords
- ectosomes
- exosomes
- extracellular vesicles
- guidelines
- microparticles
- microvesicles
- minimal information requirements
- reproducibility
- rigor
- standardization
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In: Journal of Extracellular Vesicles, Vol. 7, No. 1, 1535750, 01.01.2018.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Minimal information for studies of extracellular vesicles 2018 (MISEV2018)
T2 - a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
AU - Théry, Clotilde
AU - Witwer, Kenneth W.
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AU - Kosanović, Maja M.
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AU - O’Grady, Tina
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AU - Ochiya, Takahiro
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AU - Ortiz, Alberto
AU - Ortiz, Luis A.
AU - Osteikoetxea, Xabier
AU - Ostegaard, Ole
AU - Ostrowski, Matias
AU - Park, Jaesung
AU - Pegtel, D. Michiel
AU - Peinado, Hector
AU - Perut, Francesca
AU - Pfaffl, Michael W.
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AU - Pogge von Strandmann, Elke
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AU - Poon, Ivan K.H.
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AU - Prada, Ilaria
AU - Pulliam, Lynn
AU - Quesenberry, Peter
AU - Radeghieri, Annalisa
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AU - Raimondo, Stefania
AU - Rak, Janusz
AU - Ramirez, Marcel I.
AU - Raposo, Graça
AU - Rayyan, Morsi S.
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AU - Zarubova, Jana
AU - Žėkas, Vytautas
AU - Zhang, Jian ye
AU - Zhao, Zezhou
AU - Zheng, Lei
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N1 - Publisher Copyright: © 2018, © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
AB - The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
KW - ectosomes
KW - exosomes
KW - extracellular vesicles
KW - guidelines
KW - microparticles
KW - microvesicles
KW - minimal information requirements
KW - reproducibility
KW - rigor
KW - standardization
UR - https://www.scopus.com/pages/publications/85057143501
U2 - 10.1080/20013078.2018.1535750
DO - 10.1080/20013078.2018.1535750
M3 - Artículo
AN - SCOPUS:85057143501
SN - 2001-3078
VL - 7
JO - Journal of Extracellular Vesicles
JF - Journal of Extracellular Vesicles
IS - 1
M1 - 1535750
ER -