Molecular and cellular risk assessment of healthy human cells and cancer human cells exposed to nanoparticles

Edward Helal-Neto, Aline Oliveira da Silva de Barros, Roberta Saldanha-Gama, Renata Brandão-Costa, Luciana Magalhães Rebêlo Alencar, Clenilton Costa Dos Santos, Ramón Martínez-Máñez, Eduardo Ricci-Junior, Frank Alexis, Verônica Morandi, Christina Barja-Fidalgo, Ralph Santos-Oliveira

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Nanodrugs have in recent years been a subject of great debate. In 2017 alone, almost 50 nanodrugs were approved for clinical use worldwide. Despite the advantages related to nanodrugs/nanomedicine, there is still a lack of information regarding the biological safety, as the real behavior of these nanodrugs in the body. In order to better understand these aspects, in this study, we evaluated the effect of polylactic acid (PLA) nanoparticles (NPs) and magnetic core mesoporous silica nanoparticles (MMSN), of 1000 nm and 50 nm, respectively, on human cells. In this direction we evaluated the cell cycle, cytochemistry, proliferation and tubulogenesis on tumor cells lines: from melanoma (MV3), breast cancer (MCF-7, MDA-MB-213), glioma (U373MG), prostate (PC3), gastric (AGS) and colon adenocarcinoma (HT-29) and non-tumor cell lines: from human melanocyte (NGM), fibroblast (FGH) and endothelial (HUVEC), respectively. The data showed that an acute exposure to both, polymeric nanoparticles or MMSN, did not show any relevant toxic effects on neither tumor cells nor non-tumor cells, suggesting that although nanodrugs may present unrevealed aspects, under acute exposition to human cells they are harmless.

Original languageEnglish
Article number230
JournalInternational Journal of Molecular Sciences
Volume21
Issue number1
DOIs
StatePublished - 1 Jan 2020
Externally publishedYes

Keywords

  • Cell culture
  • Nanomedicine
  • Nanoparticles
  • Primary cells
  • Tumor cell

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