TY - CHAP
T1 - Morphometric evaluation of the spermatogonial stem cell distribution and niche in vertebrates
AU - Almeida, Paulo Henrique
AU - Jardim Costa, Guilherme Mattos
AU - De Avelar, Gleide Fernandes
AU - Segatelli, Tânia Mara
AU - Nassif Lacerda, Samyra Maria Santos
AU - Aponte, Pedro Manuel
AU - Renato De França, Luiz
PY - 2013
Y1 - 2013
N2 - Morphometry is a classical quantitative method often used in biology to provide a data basis for functional interpretations/interactions of a particular organ or system. Herein we took advantage of this valuable approach to evaluate the spermatogonial stem cell niche using the horse testis and immunocytochemical localization of GFRA1 [glial cell line-derived neurotrophic factor receptor produced by Sertoli cells)] as an example. Using the NIH ImageJ free software, we describe in detail all the necessary steps to investigate this specifi c and crucial microenvironment. Based on several recently published papers from our research group, this approach has proved to be fast, simple, and adaptable to a wide range of species and has the potential to be easily reproducible in different laboratories.
AB - Morphometry is a classical quantitative method often used in biology to provide a data basis for functional interpretations/interactions of a particular organ or system. Herein we took advantage of this valuable approach to evaluate the spermatogonial stem cell niche using the horse testis and immunocytochemical localization of GFRA1 [glial cell line-derived neurotrophic factor receptor produced by Sertoli cells)] as an example. Using the NIH ImageJ free software, we describe in detail all the necessary steps to investigate this specifi c and crucial microenvironment. Based on several recently published papers from our research group, this approach has proved to be fast, simple, and adaptable to a wide range of species and has the potential to be easily reproducible in different laboratories.
KW - GFRA1
KW - ImageJ
KW - Morphology
KW - Morphometry
KW - Niche
KW - Spermatogenesis
KW - Spermatogonial stem cell
KW - Testis
UR - https://www.scopus.com/pages/publications/84934437494
U2 - 10.1007/978-1-62703-508-8_4
DO - 10.1007/978-1-62703-508-8_4
M3 - Capítulo
C2 - 23959980
AN - SCOPUS:84934437494
SN - 9781627035071
T3 - Methods in Molecular Biology
SP - 35
EP - 42
BT - Stem Cell Niche
PB - Humana Press Inc.
ER -