Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays

Stuart Torres Ayala; Lesly Simbaña Vivanco; Nikolina Walas; Kelsey Jesser; Nicolette A. Zhou; Christine S. Fagnant-Sperati; Hadley R. Burroughs; Gwenyth O. Lee; Joseph N.S. Eisenberg; Gabriel Trueba; Karen Levy; Benjamin F. Arnold

doi:10.1093/ofid/ofaf027

Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays

Stuart Torres Ayala^*
, Lesly Simbaña Vivanco
, Nikolina Walas
, Kelsey Jesser
, Nicolette A. Zhou
, Christine S. Fagnant-Sperati
, Hadley R. Burroughs
, Gwenyth O. Lee
, Joseph N.S. Eisenberg
, Gabriel Trueba
, Karen Levy
, Benjamin F. Arnold^*

^*Corresponding author for this work

Universidad San Francisco de Quito
University of California at Berkeley
University of Washington
UCSF
Rutgers University–New Brunswick
University of Michigan School of Public Health
University of California

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We compared the performance of 2 multiplex platforms, Luminex xTAG Gastrointestinal Pathogen Panel and TaqMan Array Card, against a panel of 14 enteric pathogen targets in a community-based birth cohort in Ecuador. We found high levels of agreement and similar prevalence estimates across most pathogens.

Original language	English
Article number	ofaf027
Journal	Open Forum Infectious Diseases
Volume	12
Issue number	2
DOIs	https://doi.org/10.1093/ofid/ofaf027
State	Published - 1 Feb 2025

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

bacteria
enteric pathogens
multiplex PCR
protozoans
viruses

Access to Document

10.1093/ofid/ofaf027

Cite this

Ayala, S. T., Simbaña Vivanco, L., Walas, N., Jesser, K., Zhou, N. A., Fagnant-Sperati, C. S., Burroughs, H. R., Lee, G. O., Eisenberg, J. N. S., Trueba, G., Levy, K., & Arnold, B. F. (2025). Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays. Open Forum Infectious Diseases, 12(2), Article ofaf027. https://doi.org/10.1093/ofid/ofaf027

@article{948553acb10c4b7485f89de2b26417bf,

title = "Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays",

abstract = "We compared the performance of 2 multiplex platforms, Luminex xTAG Gastrointestinal Pathogen Panel and TaqMan Array Card, against a panel of 14 enteric pathogen targets in a community-based birth cohort in Ecuador. We found high levels of agreement and similar prevalence estimates across most pathogens.",

keywords = "bacteria, enteric pathogens, multiplex PCR, protozoans, viruses",

author = "Ayala, \{Stuart Torres\} and \{Simba{\~n}a Vivanco\}, Lesly and Nikolina Walas and Kelsey Jesser and Zhou, \{Nicolette A.\} and Fagnant-Sperati, \{Christine S.\} and Burroughs, \{Hadley R.\} and Lee, \{Gwenyth O.\} and Eisenberg, \{Joseph N.S.\} and Gabriel Trueba and Karen Levy and Arnold, \{Benjamin F.\}",

note = "Publisher Copyright: {\textcopyright} 2025 The Author(s). Published by Oxford University Press on behalf of Infectious Diseases Society of America.",

year = "2025",

month = feb,

day = "1",

doi = "10.1093/ofid/ofaf027",

language = "Ingl{\'e}s",

volume = "12",

journal = "Open Forum Infectious Diseases",

issn = "2328-8957",

publisher = "Oxford University Press",

number = "2",

}

Ayala, ST, Simbaña Vivanco, L, Walas, N, Jesser, K, Zhou, NA, Fagnant-Sperati, CS, Burroughs, HR, Lee, GO, Eisenberg, JNS, Trueba, G, Levy, K & Arnold, BF 2025, 'Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays', Open Forum Infectious Diseases, vol. 12, no. 2, ofaf027. https://doi.org/10.1093/ofid/ofaf027

TY - JOUR

T1 - Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador

T2 - Comparison of xTAG-GPP and TaqMan Array Card Assays

AU - Ayala, Stuart Torres

AU - Simbaña Vivanco, Lesly

AU - Walas, Nikolina

AU - Jesser, Kelsey

AU - Zhou, Nicolette A.

AU - Fagnant-Sperati, Christine S.

AU - Burroughs, Hadley R.

AU - Lee, Gwenyth O.

AU - Eisenberg, Joseph N.S.

AU - Trueba, Gabriel

AU - Levy, Karen

AU - Arnold, Benjamin F.

PY - 2025/2/1

Y1 - 2025/2/1

N2 - We compared the performance of 2 multiplex platforms, Luminex xTAG Gastrointestinal Pathogen Panel and TaqMan Array Card, against a panel of 14 enteric pathogen targets in a community-based birth cohort in Ecuador. We found high levels of agreement and similar prevalence estimates across most pathogens.

AB - We compared the performance of 2 multiplex platforms, Luminex xTAG Gastrointestinal Pathogen Panel and TaqMan Array Card, against a panel of 14 enteric pathogen targets in a community-based birth cohort in Ecuador. We found high levels of agreement and similar prevalence estimates across most pathogens.

KW - bacteria

KW - enteric pathogens

KW - multiplex PCR

KW - protozoans

KW - viruses

UR - https://www.scopus.com/pages/publications/85217258521

U2 - 10.1093/ofid/ofaf027

DO - 10.1093/ofid/ofaf027

M3 - Artículo

AN - SCOPUS:85217258521

SN - 2328-8957

VL - 12

JO - Open Forum Infectious Diseases

JF - Open Forum Infectious Diseases

IS - 2

M1 - ofaf027

ER -

Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays

Abstract

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this