Multiplex PCR in non-human DNA molecular identification of Ascaris spp. in forensic biology

G. Burgos; F. Yanqui-Rivera; D. Mollocana; M. Camargo; S. Zapata; V. Arahana; C. Paz-y-Miño

doi:10.1016/j.fsigss.2017.09.227

Multiplex PCR in non-human DNA molecular identification of Ascaris spp. in forensic biology

G. Burgos^*
, F. Yanqui-Rivera
, D. Mollocana
, M. Camargo
, S. Zapata
, V. Arahana
, C. Paz-y-Miño

^*Corresponding author for this work

Universidad de las Americas - Ecuador
Universidad San Francisco de Quito
Instituto Nacional de Medicina Legal y Ciencias Forenses, Colombia
Universidad Tecnológica Equinoccial

Research output: Contribution to journal › Article › peer-review

Abstract

Forensic parasitology is a promising new tool to prevent illegal trade in protected species, and other research areas like epidemiology and human public health protection. Two multiplex PCR maps for 14 microsatellites loci were designed based on information content reported in Ascaris spp. literature. PCR reactions were standardized, minimizing artefacts, to obtain genetic profiles that allowed discrimination between individuals. This protocol is an alternative to replace current STRs monoplex genotyping techniques; it permits the optimization of resources, improving specificity and sensitivity compared with common methods used to typify this parasite.

Original language	English
Pages (from-to)	e568-e569
Journal	Forensic Science International: Genetics Supplement Series
Volume	6
DOIs	https://doi.org/10.1016/j.fsigss.2017.09.227
State	Published - Dec 2017

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Ascaris spp.
DNA profiling
Forensic parasitology
Microsatellite
Molecular epidemiology
PCR multiplex

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10.1016/j.fsigss.2017.09.227

Cite this

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title = "Multiplex PCR in non-human DNA molecular identification of Ascaris spp. in forensic biology",

abstract = "Forensic parasitology is a promising new tool to prevent illegal trade in protected species, and other research areas like epidemiology and human public health protection. Two multiplex PCR maps for 14 microsatellites loci were designed based on information content reported in Ascaris spp. literature. PCR reactions were standardized, minimizing artefacts, to obtain genetic profiles that allowed discrimination between individuals. This protocol is an alternative to replace current STRs monoplex genotyping techniques; it permits the optimization of resources, improving specificity and sensitivity compared with common methods used to typify this parasite.",

keywords = "Ascaris spp., DNA profiling, Forensic parasitology, Microsatellite, Molecular epidemiology, PCR multiplex",

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T1 - Multiplex PCR in non-human DNA molecular identification of Ascaris spp. in forensic biology

AU - Burgos, G.

AU - Yanqui-Rivera, F.

AU - Mollocana, D.

AU - Camargo, M.

AU - Zapata, S.

AU - Arahana, V.

AU - Paz-y-Miño, C.

PY - 2017/12

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AB - Forensic parasitology is a promising new tool to prevent illegal trade in protected species, and other research areas like epidemiology and human public health protection. Two multiplex PCR maps for 14 microsatellites loci were designed based on information content reported in Ascaris spp. literature. PCR reactions were standardized, minimizing artefacts, to obtain genetic profiles that allowed discrimination between individuals. This protocol is an alternative to replace current STRs monoplex genotyping techniques; it permits the optimization of resources, improving specificity and sensitivity compared with common methods used to typify this parasite.

KW - Ascaris spp.

KW - DNA profiling

KW - Forensic parasitology

KW - Microsatellite

KW - Molecular epidemiology

KW - PCR multiplex

UR - https://www.scopus.com/pages/publications/85031319532

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M3 - Artículo

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SN - 1875-1768

VL - 6

SP - e568-e569

JO - Forensic Science International: Genetics Supplement Series

JF - Forensic Science International: Genetics Supplement Series

ER -

Multiplex PCR in non-human DNA molecular identification of Ascaris spp. in forensic biology

Abstract

UN SDGs

Keywords

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