NDM-1 carbapenemase in Acinetobacter baumannii sequence type 32 in Ecuador

J. E. Villacís; M. Bovera; D. Romero-Alvarez; F. Cornejo; V. Albán; G. Trueba; H. F. Dorn; J. A. Reyes

doi:10.1016/j.nmni.2019.100526

NDM-1 carbapenemase in Acinetobacter baumannii sequence type 32 in Ecuador

J. E. Villacís
, M. Bovera
, D. Romero-Alvarez
, F. Cornejo
, V. Albán
, G. Trueba
, H. F. Dorn
, J. A. Reyes^*

^*Corresponding author for this work

Pontificia Universidad Católica del Ecuador
Centro de Referencia Nacional de Resistencia a los Antimicrobianos Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”
Hospital de los Valles
University of Kansas
Universidad Central del Ecuador

Research output: Contribution to journal › Comment/debate

14 Scopus citations

Abstract

Objectives: To describe a clinical case of Acinetobacter baumannii sequence type (ST) 32 harbouring a New Delhi metallo-β-lactamase (NDM) in Ecuador. Methods: We used multilocus sequence typing (MLST) to confirm the bacterial species and the sequence type of an A. baumannii isolate. We used synergy with the imipenem–EDTA disc method and the carbapenem inactivation method (CIM) to determine carbapenemase production; the presence of a carbapenemase gene was confirmed by PCR amplification and amplicon sequencing. Results: Molecular characterization revealed the presence of A. baumannii ST32 harbouring the bla _NDM-1 gene in Ecuador. The bla _NDM-1 gene was isolated through PCR and amplified from a purified plasmid. Conclusions: To the best of our knowledge, this is the first report of A. baumannii ST32 harbouring the bla _NDM-1 gene.

Original language	English
Article number	100526
Journal	New Microbes and New Infections
Volume	29
DOIs	https://doi.org/10.1016/j.nmni.2019.100526
State	Published - May 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Acinetobacter baumannii
MSLT
antibiotic resistance
blaNDM-1
sequence type 32

Access to Document

10.1016/j.nmni.2019.100526

Cite this

@article{f0b1f3d565204080b0a3325a8f8122fe,

title = "NDM-1 carbapenemase in Acinetobacter baumannii sequence type 32 in Ecuador",

abstract = " Objectives: To describe a clinical case of Acinetobacter baumannii sequence type (ST) 32 harbouring a New Delhi metallo-β-lactamase (NDM) in Ecuador. Methods: We used multilocus sequence typing (MLST) to confirm the bacterial species and the sequence type of an A. baumannii isolate. We used synergy with the imipenem–EDTA disc method and the carbapenem inactivation method (CIM) to determine carbapenemase production; the presence of a carbapenemase gene was confirmed by PCR amplification and amplicon sequencing. Results: Molecular characterization revealed the presence of A. baumannii ST32 harbouring the bla NDM-1 gene in Ecuador. The bla NDM-1 gene was isolated through PCR and amplified from a purified plasmid. Conclusions: To the best of our knowledge, this is the first report of A. baumannii ST32 harbouring the bla NDM-1 gene.",

keywords = "Acinetobacter baumannii, MSLT, antibiotic resistance, blaNDM-1, sequence type 32",

author = "Villac{\'i}s, \{J. E.\} and M. Bovera and D. Romero-Alvarez and F. Cornejo and V. Alb{\'a}n and G. Trueba and Dorn, \{H. F.\} and Reyes, \{J. A.\}",

note = "Publisher Copyright: {\textcopyright} 2019 The Author(s)",

year = "2019",

month = may,

doi = "10.1016/j.nmni.2019.100526",

language = "Ingl{\'e}s",

volume = "29",

journal = "New Microbes and New Infections",

issn = "2052-2975",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - NDM-1 carbapenemase in Acinetobacter baumannii sequence type 32 in Ecuador

AU - Villacís, J. E.

AU - Bovera, M.

AU - Romero-Alvarez, D.

AU - Cornejo, F.

AU - Albán, V.

AU - Trueba, G.

AU - Dorn, H. F.

AU - Reyes, J. A.

PY - 2019/5

Y1 - 2019/5

N2 - Objectives: To describe a clinical case of Acinetobacter baumannii sequence type (ST) 32 harbouring a New Delhi metallo-β-lactamase (NDM) in Ecuador. Methods: We used multilocus sequence typing (MLST) to confirm the bacterial species and the sequence type of an A. baumannii isolate. We used synergy with the imipenem–EDTA disc method and the carbapenem inactivation method (CIM) to determine carbapenemase production; the presence of a carbapenemase gene was confirmed by PCR amplification and amplicon sequencing. Results: Molecular characterization revealed the presence of A. baumannii ST32 harbouring the bla NDM-1 gene in Ecuador. The bla NDM-1 gene was isolated through PCR and amplified from a purified plasmid. Conclusions: To the best of our knowledge, this is the first report of A. baumannii ST32 harbouring the bla NDM-1 gene.

AB - Objectives: To describe a clinical case of Acinetobacter baumannii sequence type (ST) 32 harbouring a New Delhi metallo-β-lactamase (NDM) in Ecuador. Methods: We used multilocus sequence typing (MLST) to confirm the bacterial species and the sequence type of an A. baumannii isolate. We used synergy with the imipenem–EDTA disc method and the carbapenem inactivation method (CIM) to determine carbapenemase production; the presence of a carbapenemase gene was confirmed by PCR amplification and amplicon sequencing. Results: Molecular characterization revealed the presence of A. baumannii ST32 harbouring the bla NDM-1 gene in Ecuador. The bla NDM-1 gene was isolated through PCR and amplified from a purified plasmid. Conclusions: To the best of our knowledge, this is the first report of A. baumannii ST32 harbouring the bla NDM-1 gene.

KW - Acinetobacter baumannii

KW - MSLT

KW - antibiotic resistance

KW - blaNDM-1

KW - sequence type 32

UR - https://www.scopus.com/pages/publications/85063330193

U2 - 10.1016/j.nmni.2019.100526

DO - 10.1016/j.nmni.2019.100526

M3 - Comentario/Debate

AN - SCOPUS:85063330193

SN - 2052-2975

VL - 29

JO - New Microbes and New Infections

JF - New Microbes and New Infections

M1 - 100526

ER -

NDM-1 carbapenemase in Acinetobacter baumannii sequence type 32 in Ecuador

Abstract

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this