New Bacteriophages Members of the Ackermannviridae Family Specific for Klebsiella pneumoniae ST258

Estefanía Tisalema-Guanopatín, Fausto Cabezas-Mera, Karla Nolivos-Rodríguez, Isabel Fierro, Lourdes Pazmiño, Daniel Garzon-Chavez, Alexis Debut, Karla Vizuete, Jorge Aníbal Reyes

Research output: Contribution to journalArticlepeer-review


Background: Carbapenem-resistant Klebsiella pneumoniae, particularly isolates classified as sequence-type 258 (ST258), are multidrug-resistant strains that are strongly associated with poor-prognosis nosocomial infections, as current therapeutic options are limited and ineffective. In recent years, phage therapy has emerged as a promising treatment option for these scenarios. Methodology and Results: We report the isolation and characterization of three new phages against Klebsiella pneumoniae ST258 strains recovered from Machángara river wastewater. These new members of the Ackermannviridae family showed stability over a wide temperature and pH range and burst sizes ranging from 6 to 44 plaque-forming units per bacteria. Their genomes were about 157 kilobases, with an average guanine-cytosine content of 46.4% and showed presence of several transfer RNAs, which also allowed us to predict in silico a lytic replicative cycle due to the presence of endolysins and lysozymes. Conclusion: Three lytic phages of Ackermannviridae family were recovered against Klebsiella pneumoniae ST258 strains from sewage; however, further characterization is needed for future consideration as therapeutic alternatives.

Original languageEnglish
Pages (from-to)99-107
Number of pages9
JournalPHAGE: Therapy, Applications, and Research
Issue number2
StatePublished - 1 Jun 2023


  • Ackermannviridae
  • Klebsiella pneumoniae
  • ST258
  • lytic
  • tRNA
  • wastewater


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