Novel serotype of bluetongue virus in South America and first report of epizootic haemorrhagic disease virus in Ecuador

J. Verdezoto; E. Breard; C. Viarouge; H. Quenault; P. Lucas; C. Sailleau; S. Zientara; D. Augot; S. Zapata

doi:10.1111/tbed.12625

Novel serotype of bluetongue virus in South America and first report of epizootic haemorrhagic disease virus in Ecuador

J. Verdezoto
, E. Breard
, C. Viarouge
, H. Quenault
, P. Lucas
, C. Sailleau
, S. Zientara
, D. Augot
, S. Zapata^*

^*Corresponding author for this work

Universidad San Francisco de Quito
Université Paris-Est ANSES Alfort
Laboratory of Ploufragan
Université de Reims Champagne-Ardenne

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Bluetongue virus (BTV) and Epizootic haemorrhagic disease virus (EHDV) are closely related Orbiviruses that affect domestic and wild ruminants. In Ecuador previous serological studies reported the presence of BTV; however, no data are available about the presence of EHDV. In this study, 295 cattle without symptoms of infection were sampled from two farms located in Andean and Amazonian regions and from a slaughterhouse in the coastal region. ELISA analyses showed high prevalence of BTV (98.9%) and EHDV (81.3%) antibodies, and RT-qPCRs revealed the presence of EHDV (24.1%) and BTV (10.2%) genomes in cattle blood samples. Viral isolation allowed to identify EHDV serotype 1 (EHDV1) and BTV serotypes 9 (BTV9), 13 and 18. These findings suggest that BTV and EHDV are enzootic diseases in Ecuador.

Original language	English
Pages (from-to)	244-247
Number of pages	4
Journal	Transboundary and Emerging Diseases
Volume	65
Issue number	1
DOIs	https://doi.org/10.1111/tbed.12625
State	Published - Feb 2018

Keywords

Bluetongue
Ecuador
Orbivirus
cattle
epizootic haemorrhagic disease

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10.1111/tbed.12625

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title = "Novel serotype of bluetongue virus in South America and first report of epizootic haemorrhagic disease virus in Ecuador",

abstract = "Bluetongue virus (BTV) and Epizootic haemorrhagic disease virus (EHDV) are closely related Orbiviruses that affect domestic and wild ruminants. In Ecuador previous serological studies reported the presence of BTV; however, no data are available about the presence of EHDV. In this study, 295 cattle without symptoms of infection were sampled from two farms located in Andean and Amazonian regions and from a slaughterhouse in the coastal region. ELISA analyses showed high prevalence of BTV (98.9\%) and EHDV (81.3\%) antibodies, and RT-qPCRs revealed the presence of EHDV (24.1\%) and BTV (10.2\%) genomes in cattle blood samples. Viral isolation allowed to identify EHDV serotype 1 (EHDV1) and BTV serotypes 9 (BTV9), 13 and 18. These findings suggest that BTV and EHDV are enzootic diseases in Ecuador.",

keywords = "Bluetongue, Ecuador, Orbivirus, cattle, epizootic haemorrhagic disease",

author = "J. Verdezoto and E. Breard and C. Viarouge and H. Quenault and P. Lucas and C. Sailleau and S. Zientara and D. Augot and S. Zapata",

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year = "2018",

month = feb,

doi = "10.1111/tbed.12625",

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TY - JOUR

T1 - Novel serotype of bluetongue virus in South America and first report of epizootic haemorrhagic disease virus in Ecuador

AU - Verdezoto, J.

AU - Breard, E.

AU - Viarouge, C.

AU - Quenault, H.

AU - Lucas, P.

AU - Sailleau, C.

AU - Zientara, S.

AU - Augot, D.

AU - Zapata, S.

PY - 2018/2

Y1 - 2018/2

N2 - Bluetongue virus (BTV) and Epizootic haemorrhagic disease virus (EHDV) are closely related Orbiviruses that affect domestic and wild ruminants. In Ecuador previous serological studies reported the presence of BTV; however, no data are available about the presence of EHDV. In this study, 295 cattle without symptoms of infection were sampled from two farms located in Andean and Amazonian regions and from a slaughterhouse in the coastal region. ELISA analyses showed high prevalence of BTV (98.9%) and EHDV (81.3%) antibodies, and RT-qPCRs revealed the presence of EHDV (24.1%) and BTV (10.2%) genomes in cattle blood samples. Viral isolation allowed to identify EHDV serotype 1 (EHDV1) and BTV serotypes 9 (BTV9), 13 and 18. These findings suggest that BTV and EHDV are enzootic diseases in Ecuador.

AB - Bluetongue virus (BTV) and Epizootic haemorrhagic disease virus (EHDV) are closely related Orbiviruses that affect domestic and wild ruminants. In Ecuador previous serological studies reported the presence of BTV; however, no data are available about the presence of EHDV. In this study, 295 cattle without symptoms of infection were sampled from two farms located in Andean and Amazonian regions and from a slaughterhouse in the coastal region. ELISA analyses showed high prevalence of BTV (98.9%) and EHDV (81.3%) antibodies, and RT-qPCRs revealed the presence of EHDV (24.1%) and BTV (10.2%) genomes in cattle blood samples. Viral isolation allowed to identify EHDV serotype 1 (EHDV1) and BTV serotypes 9 (BTV9), 13 and 18. These findings suggest that BTV and EHDV are enzootic diseases in Ecuador.

KW - Bluetongue

KW - Ecuador

KW - Orbivirus

KW - cattle

KW - epizootic haemorrhagic disease

UR - https://www.scopus.com/pages/publications/85013806178

U2 - 10.1111/tbed.12625

DO - 10.1111/tbed.12625

M3 - Artículo

C2 - 28239988

AN - SCOPUS:85013806178

SN - 1865-1674

VL - 65

SP - 244

EP - 247

JO - Transboundary and Emerging Diseases

JF - Transboundary and Emerging Diseases

IS - 1

ER -

Novel serotype of bluetongue virus in South America and first report of epizootic haemorrhagic disease virus in Ecuador

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Keywords

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