Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells

Francesca Giampieri; Massimiliano Gasparrini; Tamara Y. Forbes-Hernandez; Luca Mazzoni; Franco Capocasa; Silvia Sabbadini; Josè M. Alvarez-Suarez; Sadia Afrin; Carlo Rosati; Tiziana Pandolfini; Barbara Molesini; José F. Sánchez-Sevilla; Iraida Amaya; Bruno Mezzetti; Maurizio Battino

doi:10.1021/acs.jafc.7b04177

Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells

Francesca Giampieri
, Massimiliano Gasparrini
, Tamara Y. Forbes-Hernandez
, Luca Mazzoni
, Franco Capocasa
, Silvia Sabbadini
, Josè M. Alvarez-Suarez
, Sadia Afrin
, Carlo Rosati
, Tiziana Pandolfini
, Barbara Molesini
, José F. Sánchez-Sevilla
, Iraida Amaya
, Bruno Mezzetti
, Maurizio Battino^*

^*Corresponding author for this work

Universita Politecnica Delle Marche
Universidad Internacional Iberoamericana (UNINI)
Universidad de las Americas - Ecuador
Agenzia nazionale per le nuove tecnologie, l'energia e lo sviluppo economico sostenibile
University of Verona
Instituto de Investigacion y Formacion Agraria y Pesquera
Universidad Europea Del Atlántico (UEA)

Research output: Contribution to journal › Article › peer-review

100 Scopus citations

Abstract

Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.

Original language	English
Pages (from-to)	581-592
Number of pages	12
Journal	Journal of Agricultural and Food Chemistry
Volume	66
Issue number	3
DOIs	https://doi.org/10.1021/acs.jafc.7b04177
State	Published - 24 Jan 2018
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

RNA-seq
ROS
anthocyanidin synthase
anticancer effects
apoptosis
flavonoids
mitochondrial functionality
strawberry
transcriptome

Access to Document

10.1021/acs.jafc.7b04177

Cite this

Giampieri, F., Gasparrini, M., Forbes-Hernandez, T. Y., Mazzoni, L., Capocasa, F., Sabbadini, S., Alvarez-Suarez, J. M., Afrin, S., Rosati, C., Pandolfini, T., Molesini, B., Sánchez-Sevilla, J. F., Amaya, I., Mezzetti, B., & Battino, M. (2018). Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells. Journal of Agricultural and Food Chemistry, 66(3), 581-592. https://doi.org/10.1021/acs.jafc.7b04177

@article{c9306294ca894137a542039d128adbd0,

title = "Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells",

abstract = "Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.",

keywords = "RNA-seq, ROS, anthocyanidin synthase, anticancer effects, apoptosis, flavonoids, mitochondrial functionality, strawberry, transcriptome",

author = "Francesca Giampieri and Massimiliano Gasparrini and Forbes-Hernandez, \{Tamara Y.\} and Luca Mazzoni and Franco Capocasa and Silvia Sabbadini and Alvarez-Suarez, \{Jos{\`e} M.\} and Sadia Afrin and Carlo Rosati and Tiziana Pandolfini and Barbara Molesini and S{\'a}nchez-Sevilla, \{Jos{\'e} F.\} and Iraida Amaya and Bruno Mezzetti and Maurizio Battino",

note = "Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

year = "2018",

month = jan,

day = "24",

doi = "10.1021/acs.jafc.7b04177",

language = "Ingl{\'e}s",

volume = "66",

pages = "581--592",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

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}

Giampieri, F, Gasparrini, M, Forbes-Hernandez, TY, Mazzoni, L, Capocasa, F, Sabbadini, S, Alvarez-Suarez, JM, Afrin, S, Rosati, C, Pandolfini, T, Molesini, B, Sánchez-Sevilla, JF, Amaya, I, Mezzetti, B & Battino, M 2018, 'Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells', Journal of Agricultural and Food Chemistry, vol. 66, no. 3, pp. 581-592. https://doi.org/10.1021/acs.jafc.7b04177

Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells. / Giampieri, Francesca; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y. et al.
In: Journal of Agricultural and Food Chemistry, Vol. 66, No. 3, 24.01.2018, p. 581-592.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells

AU - Giampieri, Francesca

AU - Gasparrini, Massimiliano

AU - Forbes-Hernandez, Tamara Y.

AU - Mazzoni, Luca

AU - Capocasa, Franco

AU - Sabbadini, Silvia

AU - Alvarez-Suarez, Josè M.

AU - Afrin, Sadia

AU - Rosati, Carlo

AU - Pandolfini, Tiziana

AU - Molesini, Barbara

AU - Sánchez-Sevilla, José F.

AU - Amaya, Iraida

AU - Mezzetti, Bruno

AU - Battino, Maurizio

PY - 2018/1/24

Y1 - 2018/1/24

N2 - Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.

AB - Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.

KW - RNA-seq

KW - ROS

KW - anthocyanidin synthase

KW - anticancer effects

KW - apoptosis

KW - flavonoids

KW - mitochondrial functionality

KW - strawberry

KW - transcriptome

UR - https://www.scopus.com/pages/publications/85041119591

U2 - 10.1021/acs.jafc.7b04177

DO - 10.1021/acs.jafc.7b04177

M3 - Artículo

C2 - 29291263

AN - SCOPUS:85041119591

SN - 0021-8561

VL - 66

SP - 581

EP - 592

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 3

ER -

Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells

Abstract

UN SDGs

Keywords

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