TY - JOUR
T1 - Tracking sars-cov-2
T2 - Novel trends and diagnostic strategies
AU - Guaman-Bautista, Linda P.
AU - Moreta-Urbano, Erick
AU - Oña-Arias, Claudia G.
AU - Torres-Arias, Marbel
AU - Kyriakidis, Nikolaos C.
AU - Malcı, Koray
AU - Jonguitud-Borrego, Nestor
AU - Rios-Solis, Leonardo
AU - Ramos-Martinez, Espiridion
AU - López-Cortés, Andrés
AU - Barba-Ostria, Carlos
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/11
Y1 - 2021/11
N2 - The COVID-19 pandemic has had an enormous impact on economies and health systems globally, therefore a top priority is the development of increasingly better diagnostic and surveillance alternatives to slow down the spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In order to establish massive testing and contact tracing policies, it is crucial to have a clear view of the diagnostic options available and their principal advantages and drawbacks. Although classical molecular methods such as RT-qPCR are broadly used, diagnostic alternatives based on technologies such as LAMP, antigen, serological testing, or the application of novel technologies such as CRISPR-Cas for diagnostics, are also discussed. The present review also discusses the most important automation strategies employed to increase testing capability. Several serological-based diagnostic kits are presented, as well as novel nanotechnology-based diagnostic methods. In summary, this review provides a clear diagnostic landscape of the most relevant tools to track COVID-19.
AB - The COVID-19 pandemic has had an enormous impact on economies and health systems globally, therefore a top priority is the development of increasingly better diagnostic and surveillance alternatives to slow down the spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In order to establish massive testing and contact tracing policies, it is crucial to have a clear view of the diagnostic options available and their principal advantages and drawbacks. Although classical molecular methods such as RT-qPCR are broadly used, diagnostic alternatives based on technologies such as LAMP, antigen, serological testing, or the application of novel technologies such as CRISPR-Cas for diagnostics, are also discussed. The present review also discusses the most important automation strategies employed to increase testing capability. Several serological-based diagnostic kits are presented, as well as novel nanotechnology-based diagnostic methods. In summary, this review provides a clear diagnostic landscape of the most relevant tools to track COVID-19.
KW - Antigen testing
KW - Automation
KW - COVID-19
KW - CRISPR-based diagnostics
KW - Nanotechnology-based diagnostics
KW - Nucleic acid amplification test
UR - http://www.scopus.com/inward/record.url?scp=85118856722&partnerID=8YFLogxK
U2 - 10.3390/diagnostics11111981
DO - 10.3390/diagnostics11111981
M3 - Artículo de revisión
AN - SCOPUS:85118856722
SN - 2075-4418
VL - 11
JO - Diagnostics
JF - Diagnostics
IS - 11
M1 - 1981
ER -