Fluidized beds are used in a variety of process industries because they provide uniform temperature distributions, low pressure drops, and high heat/mass rates. Minimum fluidization velocity is an important factor in understanding the hydrodynamic behavior of fluidized beds, and this characteristic may be modified through high frequency (sound) vibrations. The effects caused by sound wave frequency on the minimum fluidization velocity in a 3D fluidized bed are investigated in this study. Experiments are carried out in a 10.2 cm ID cold flow fluidized bed filled with glass beads with material density of 2600 kg/m3, and particles sizes ranging between 212-600 μm. In this study, four different bed height-to-diameter ratios are examined: H/D = 0.5, 1, 1.5, and 2. Moreover, the sound frequency of the loudspeaker used as the acoustic source ranges between 50-200 Hz, with a sound pressure level fixed at 110 dB. Results show that the minimum fluidization velocity is influenced by the frequency change. As the frequency increases, the minimum fluidization velocity decreases until a specific frequency is reached, beyond which the minimum fluidization velocity increases. Thus, acoustic fields provide an improvement in the ease of fluidization of these particles.