Activation of AMPK/Nrf2 signalling by Manuka honey protects human dermal fibroblasts against oxidative damage by improving antioxidant response and mitochondrial function promoting wound healing

José Miguel Alvarez-Suarez, Francesca Giampieri, Mario Cordero, Massimiliano Gasparrini, Tamara Yuliet Forbes-Hernández, Luca Mazzoni, Sadia Afrin, Pablo Beltrán-Ayala, Ana María González-Paramás, Celestino Santos-Buelga, Alfonso Varela-Lopez, José Luis Quiles, Maurizio Battino

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

128 Citas (Scopus)

Resumen

Excessive amounts of free radicals are deleterious for cells, resulting in cell damage, affecting the wound healing process and causing premature ageing or even neoplastic transformation. Here the capacity of Manuka honey (MH) to protect against oxidative damage and improve the process of skin wound healing was investigated. Up to 16 compounds were identified in MH, with leptosin derivatives and methyl syringate as the major ones. MH protected against apoptosis, intracellular ROS production, and lipid and protein oxidative damage. MH also protected mitochondrial functionality, promoted cell proliferation and activated the AMPK/Nrf2/ARE signalling pathway, as well as the expression of the antioxidant enzymes such as SOD and CAT. Here we describe for the first time that one of the possible mechanisms by which MH exhibits its ability to promote wound healing could be due to its capacity to improve the antioxidant response by activating AMPK phosphorylation and the ARE response.

Idioma originalInglés
Páginas (desde-hasta)38-49
Número de páginas12
PublicaciónJournal of Functional Foods
Volumen25
DOI
EstadoPublicada - 1 ago. 2016

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