TY - JOUR
T1 - Anti-inflammatory activities of Italian Chestnut and Eucalyptus honeys on murine RAW 264.7 macrophages
AU - Cianciosi, Danila
AU - Forbes-Hernandez, Tamara Y.
AU - Alvarez-Suarez, José M.
AU - Ansary, Johura
AU - Quinzi, Denise
AU - Amici, Adolfo
AU - Navarro-Hortal, María D.
AU - Esteban-Muñoz, Adelaida
AU - Quiles, José Luis
AU - Battino, Maurizio
AU - Giampieri, Francesca
N1 - Publisher Copyright:
© 2021
PY - 2021/12
Y1 - 2021/12
N2 - The aim of this study was to evaluate for the first time the anti-inflammatory effects of chestnut (Castanea sativa) and Eucalyptus (Eucalyptus occidentalis) honeys collected in Italy on murine RAW 264.7 macrophages stressed with the bacterial endotoxin lipopolysaccharide (LPS). Pre-treatment with the two honeys markedly decreased apoptosis and stimulated wound healing in macrophages stressed with LPS, where they also reduced the intracellular levels of ROS and nitrite. Additionally, treatment with LPS decreased the levels of glutathione and suppressed the antioxidant enzyme activities, which were significantly (p < 0.05) increased in the presence of the two honeys, through the modulation of Nrf2 expression; in LPS-stressed macrophages, Chestnut or Eucalyptus honeys protected against inflammation by regulating the main inflammatory biomarkers, including TNF-α, IL-10 and iNOS, through the decrease of NF-кB expression. Finally, they improved mitochondrial respiration and the main related parameters.
AB - The aim of this study was to evaluate for the first time the anti-inflammatory effects of chestnut (Castanea sativa) and Eucalyptus (Eucalyptus occidentalis) honeys collected in Italy on murine RAW 264.7 macrophages stressed with the bacterial endotoxin lipopolysaccharide (LPS). Pre-treatment with the two honeys markedly decreased apoptosis and stimulated wound healing in macrophages stressed with LPS, where they also reduced the intracellular levels of ROS and nitrite. Additionally, treatment with LPS decreased the levels of glutathione and suppressed the antioxidant enzyme activities, which were significantly (p < 0.05) increased in the presence of the two honeys, through the modulation of Nrf2 expression; in LPS-stressed macrophages, Chestnut or Eucalyptus honeys protected against inflammation by regulating the main inflammatory biomarkers, including TNF-α, IL-10 and iNOS, through the decrease of NF-кB expression. Finally, they improved mitochondrial respiration and the main related parameters.
KW - Antioxidant enzymes
KW - Apoptosis
KW - Honey
KW - Inflammation
KW - Mitochondrial respiration
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=85114909981&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2021.104752
DO - 10.1016/j.jff.2021.104752
M3 - Artículo
AN - SCOPUS:85114909981
SN - 1756-4646
VL - 87
JO - Journal of Functional Foods
JF - Journal of Functional Foods
M1 - 104752
ER -