Cloning of the pfaP gene of Leptospira borgpetersenii

Gabriel A. Trueba; Carole A. Bolin; Richard L. Zuerner

doi:10.1016/0378-1119(95)00201-G

Cloning of the pfaP gene of Leptospira borgpetersenii

Gabriel A. Trueba, Carole A. Bolin, Richard L. Zuerner

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

2 Citas (Scopus)

Resumen

A λgtll library constructed with Leptospira borgpetersenii DNA was screened with monoclonal antibodies (mAb) recognizing a periplasmic flagella-associated protein. A plaque expressing a fusion protein (λF15) which reacted with the mAb was isolated and the nucleotide sequence analyzed. The deduced amino-acid (aa) sequence indicates that the pfaP gene belongs to a group of bacterial genes whose products share aa sequence and possibly functional homologies with sppA, an Escherichia coli signal peptidase-encoding gene.

Idioma original	Inglés
Páginas (desde-hasta)	133-134
Número de páginas	2
Publicación	Gene
Volumen	160
N.º	1
DOI	https://doi.org/10.1016/0378-1119(95)00201-G
Estado	Publicada - 4 jul. 1995
Publicado de forma externa	Sí

Acceder al documento

10.1016/0378-1119(95)00201-G

Otros archivos y enlaces

Enlace a la publicación en Scopus

Citar esto

@article{2d5c80344a3442689c7d873a0747f260,

title = "Cloning of the pfaP gene of Leptospira borgpetersenii",

abstract = "A λgtll library constructed with Leptospira borgpetersenii DNA was screened with monoclonal antibodies (mAb) recognizing a periplasmic flagella-associated protein. A plaque expressing a fusion protein (λF15) which reacted with the mAb was isolated and the nucleotide sequence analyzed. The deduced amino-acid (aa) sequence indicates that the pfaP gene belongs to a group of bacterial genes whose products share aa sequence and possibly functional homologies with sppA, an Escherichia coli signal peptidase-encoding gene.",

keywords = "Recombinant DNA, pathogen, periplasmic flagella, signal peptidase, spirochete, sppA",

author = "Trueba, {Gabriel A.} and Bolin, {Carole A.} and Zuerner, {Richard L.}",

year = "1995",

month = jul,

day = "4",

doi = "10.1016/0378-1119(95)00201-G",

language = "Ingl{\'e}s",

volume = "160",

pages = "133--134",

journal = "Gene",

issn = "0378-1119",

number = "1",

}

TY - JOUR

T1 - Cloning of the pfaP gene of Leptospira borgpetersenii

AU - Trueba, Gabriel A.

AU - Bolin, Carole A.

AU - Zuerner, Richard L.

PY - 1995/7/4

Y1 - 1995/7/4

N2 - A λgtll library constructed with Leptospira borgpetersenii DNA was screened with monoclonal antibodies (mAb) recognizing a periplasmic flagella-associated protein. A plaque expressing a fusion protein (λF15) which reacted with the mAb was isolated and the nucleotide sequence analyzed. The deduced amino-acid (aa) sequence indicates that the pfaP gene belongs to a group of bacterial genes whose products share aa sequence and possibly functional homologies with sppA, an Escherichia coli signal peptidase-encoding gene.

AB - A λgtll library constructed with Leptospira borgpetersenii DNA was screened with monoclonal antibodies (mAb) recognizing a periplasmic flagella-associated protein. A plaque expressing a fusion protein (λF15) which reacted with the mAb was isolated and the nucleotide sequence analyzed. The deduced amino-acid (aa) sequence indicates that the pfaP gene belongs to a group of bacterial genes whose products share aa sequence and possibly functional homologies with sppA, an Escherichia coli signal peptidase-encoding gene.

KW - Recombinant DNA

KW - pathogen

KW - periplasmic flagella

KW - signal peptidase

KW - spirochete

KW - sppA

UR - http://www.scopus.com/inward/record.url?scp=0029025237&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(95)00201-G

DO - 10.1016/0378-1119(95)00201-G

M3 - Artículo

C2 - 7628708

AN - SCOPUS:0029025237

SN - 0378-1119

VL - 160

SP - 133

EP - 134

JO - Gene

JF - Gene

IS - 1

ER -

Cloning of the pfaP gene of Leptospira borgpetersenii

Resumen

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto