Design of a biomimetic, 3d scaffold suitable for different tissue engineering applications

Jose F. Alvarez-Barreto, Jessica Yankovich, Mark C. Shreve, Paul L. DeAngelis, Vassilios I. Sikavitsas

Producción científica: Contribución a una conferenciaArtículorevisión exhaustiva


Scaffolds for tissue engineering have been created using a wide variety of techniques and materials. Poly (alpha-hydroxy esters) are widely used due to their degradation characteristics and mechanical properties. Nevertheless, these polymers posses a hydrophobic and inert nature that affects cell-matrix interactions. Thus, the creation of a construct that can mimic the in vivo molecular environment is crucial for the formation of efficient constructs for tissue engineering. In this study, we prepared a flexible, biomimetic 3D scaffold by entrapping poly (L-lysine) (PLL) in the surface of poly(L-lactic acid) (PLLA) foams in a controllable fashion. PLLA scaffolds were made by particulate leaching, using sodium chloride as the porogen. Poly (L-lysine) was physically entrapped in the surface by soaking the scaffold in acetone, and then in a solution of PLL under vacuum. N-Succinimidyl-3-(2-pyridyldithio) propionate (SPDP) was attached to the PLL and activated with dithiothreitol (DTT) for further incorporation of fluorescein-5-maleimide. Fluorescence microscopy showed a homogeneous distribution of the PLL throughout the surface of the scaffold porous network. We were also able to control the amount of entrapped PLL. By using different linkers, such as degradable polypeptides, this stable modification can be used to attach different molecules specific for a wide array of tissue engineering applications. We have been able to incorporate an arginine-glysine-aspartic acid-cysteine (RGDC) sequence by modifying the PLL with an NHS ester. X-ray photo spectroscopy corroborated the presence of the RGDC on the surface pf the scaffolds. Mesemchymal stem cells (MSC) were seeded on the RGD-modified surface (unmodified scaffolds were used as controls). Enhanced cell attachment was observed on RGD-modified surfaces, with cells showing improved cell-matrix interactions translated into better cell spreading and higher number of cells attached.

Idioma originalInglés
Número de páginas1
EstadoPublicada - 2005
Evento05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, Estados Unidos
Duración: 30 oct. 20054 nov. 2005


Conferencia05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
País/TerritorioEstados Unidos
CiudadCincinnati, OH


Profundice en los temas de investigación de 'Design of a biomimetic, 3d scaffold suitable for different tissue engineering applications'. En conjunto forman una huella única.

Citar esto