Direct and indirect antioxidant activity of polyphenol- and isothiocyanate-enriched fractions from moringa oleifera

Tugba Boyunegmez Tumer, Patricio Rojas-Silva, Alexander Poulev, Ilya Raskin, Carrie Waterman

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

108 Citas (Scopus)

Resumen

Moringa oleifera Lam. is a fast-growing, tropical tree with various edible parts used as nutritious food and traditional medicine. This study describes an efficient preparatory strategy to extract and fractionate moringa leaves by fast centrifugal partition chromatography (FCPC) to produce polyphenol and isothiocyanate (ITC) rich fractions. Characterization and further purification of these fractions showed that moringa polyphenols were potent direct antioxidants assayed by oxygen radical absorbance capacity (ORAC), whereas moringa ITCs were effective indirect antioxidants assayed by induction of NAD(P)H quinone oxidoreductase 1 (NQO1) activity in Hepa1c1c7 cells. In addition, purified 4-[(α-l-rhamnosyloxy)benzyl]isothiocyanate and 4-[(4′-O-acetyl-α-l-rhamnosyloxy)benzyl]isothiocyanate were further evaluated for their ORAC and NQO1 inducer potency in comparison with sulforaphane (SF). Both ITCs were as potent as SF in inducing NQO1 activity. These findings suggest that moringa leaves contain a potent mixture of direct and indirect antioxidants that can explain its various health-promoting effects.

Idioma originalInglés
Páginas (desde-hasta)1505-1513
Número de páginas9
PublicaciónJournal of Agricultural and Food Chemistry
Volumen63
N.º5
DOI
EstadoPublicada - 11 feb. 2015
Publicado de forma externa

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