IgG1/IgG2 antibody dichotomy in sera of vaccinated or naturally infected dogs with visceral leishmaniosis

Carolina De Oliveira Mendes, Edilma Paraguai De Souza, Gulnara Patrícia Borja-Cabrera, Leopoldina Maria Melo Batista, Miriam Aparecida Dos Santos, Luciana Ellner Parra, Ingrid Menz, Marcos Palatnik, Clarisa Beatriz Palatnik De Sousa

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

60 Citas (Scopus)

Resumen

Canine antibody IgG, IgG1 and IgG2 anti-FML responses were investigated in dogs vaccinated with the fucose-mannose ligand (FML)-vaccine of Leishmania donovani and in dogs with naturally acquired visceral leishmaniosis. While similar levels of total IgG antibodies were seen in the seropositive naturally infected dogs and in vaccinees, significant differences between the groups were found regarding their IgG1/IgG2 anti-FML antibody composition (P<0.005). Higher IgG1 absorbencies were seen in infected dogs, while the IgG2 subtype was predominant in pre-immune sera, and in vaccinated animals, both after the first and the third dose (P<0.005). The average ratio between IgG1/IgG2 was then 1.124 for infected animals and 0.733 for FML-vaccinees. Also, a significant increase in IgG2 antibodies was observed from the first to the third vaccine injection (P<0.005). In the infected dogs, a high correlation between their IgG absorbance (Abs) values and the number of symptoms (P=0.017) was disclosed. Thus, the analysis of IgG subclasses disclosed a dichotomous response to visceral leishmaniosis: IgG1 associated to natural infection and IgG2 associated to a humoral response subsequent to the FML-vaccine treatment. An IgG1/IgG2≥1 would characterize the sera of visceral leishmaniasis infected animals evoluting towards the overt disease while ratios ≤1 would characterize the sera response of vaccinated protected dogs.

Idioma originalInglés
Páginas (desde-hasta)2589-2597
Número de páginas9
PublicaciónVaccine
Volumen21
N.º19-20
DOI
EstadoPublicada - 2 jun. 2003
Publicado de forma externa

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