Morphology, ultrastructure, and small subunit rDNA phylogeny of the marine heterotrophic flagellate goniomonas aff. amphinema

Mercedes Martin-Cereceda, Emily C. Roberts, Emma C. Wootton, Elisa Bonaccorso, Patricia Dyal, Almudena Guinea, Dale Rogers, Chris J. Wright, Gianfranco Novarino

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

13 Citas (Scopus)

Resumen

Marine goniomonads have a worldwide distribution but ultrastructural information has not been available so far. An isolate of the heterotrophic marine nanoflagellate Goniomonas (G. aff. amphinema) from North Wales (UK) has been studied, providing information on its morphology and cellular structure using video, electron, laser scanning confocal microscopy (LSCM), and atomic force microscopy. Here, we describe a new feature, a granular area, potentially involved in particle capture and feeding. The binding of the lectin wheat germ agglutinin to the granular area of cells with discharged ejectisomes indicates the adhesive nature of this novel feature. The presence of a microtubular intracellular cytopharynx, apparently also used for feeding, has been revealed by LSCM. The small subunit rRNA gene of the isolate has been sequenced (1,788 bp). Phylogenetic results corroborate significant genetic divergence within the marine members of Goniomonas. This work highlights the need for integrated morphological, ultrastructural, and molecular investigation when describing and studying heterotrophic nanoflagellates.

Idioma originalInglés
Páginas (desde-hasta)159-170
Número de páginas12
PublicaciónJournal of Eukaryotic Microbiology
Volumen57
N.º2
DOI
EstadoPublicada - mar. 2010
Publicado de forma externa

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