Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays

Stuart Torres Ayala; Lesly Simbaña Vivanco; Nikolina Walas; Kelsey Jesser; Nicolette A. Zhou; Christine S. Fagnant-Sperati; Hadley R. Burroughs; Gwenyth O. Lee; Joseph N.S. Eisenberg; Gabriel Trueba; Karen Levy; Benjamin F. Arnold

doi:10.1093/ofid/ofaf027

Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays

Stuart Torres Ayala^*
, Lesly Simbaña Vivanco
, Nikolina Walas
, Kelsey Jesser
, Nicolette A. Zhou
, Christine S. Fagnant-Sperati
, Hadley R. Burroughs
, Gwenyth O. Lee
, Joseph N.S. Eisenberg
, Gabriel Trueba
, Karen Levy
, Benjamin F. Arnold^*

^*Autor correspondiente de este trabajo

Universidad San Francisco de Quito
University of California at Berkeley
University of Washington
UCSF
Rutgers University–New Brunswick
University of Michigan School of Public Health
University of California

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Resumen

We compared the performance of 2 multiplex platforms, Luminex xTAG Gastrointestinal Pathogen Panel and TaqMan Array Card, against a panel of 14 enteric pathogen targets in a community-based birth cohort in Ecuador. We found high levels of agreement and similar prevalence estimates across most pathogens.

Idioma original	Inglés
Número de artículo	ofaf027
Publicación	Open Forum Infectious Diseases
Volumen	12
N.º	2
DOI	https://doi.org/10.1093/ofid/ofaf027
Estado	Publicada - 1 feb. 2025

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Ayala, S. T., Simbaña Vivanco, L., Walas, N., Jesser, K., Zhou, N. A., Fagnant-Sperati, C. S., Burroughs, H. R., Lee, G. O., Eisenberg, J. N. S., Trueba, G., Levy, K., & Arnold, B. F. (2025). Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays. Open Forum Infectious Diseases, 12(2), Artículo ofaf027. https://doi.org/10.1093/ofid/ofaf027

@article{948553acb10c4b7485f89de2b26417bf,

title = "Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays",

abstract = "We compared the performance of 2 multiplex platforms, Luminex xTAG Gastrointestinal Pathogen Panel and TaqMan Array Card, against a panel of 14 enteric pathogen targets in a community-based birth cohort in Ecuador. We found high levels of agreement and similar prevalence estimates across most pathogens.",

keywords = "bacteria, enteric pathogens, multiplex PCR, protozoans, viruses",

author = "Ayala, \{Stuart Torres\} and \{Simba{\~n}a Vivanco\}, Lesly and Nikolina Walas and Kelsey Jesser and Zhou, \{Nicolette A.\} and Fagnant-Sperati, \{Christine S.\} and Burroughs, \{Hadley R.\} and Lee, \{Gwenyth O.\} and Eisenberg, \{Joseph N.S.\} and Gabriel Trueba and Karen Levy and Arnold, \{Benjamin F.\}",

note = "Publisher Copyright: {\textcopyright} 2025 The Author(s). Published by Oxford University Press on behalf of Infectious Diseases Society of America.",

year = "2025",

month = feb,

day = "1",

doi = "10.1093/ofid/ofaf027",

language = "Ingl{\'e}s",

volume = "12",

journal = "Open Forum Infectious Diseases",

issn = "2328-8957",

publisher = "Oxford University Press",

number = "2",

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Ayala, ST, Simbaña Vivanco, L, Walas, N, Jesser, K, Zhou, NA, Fagnant-Sperati, CS, Burroughs, HR, Lee, GO, Eisenberg, JNS, Trueba, G, Levy, K & Arnold, BF 2025, 'Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays', Open Forum Infectious Diseases, vol. 12, n.º 2, ofaf027. https://doi.org/10.1093/ofid/ofaf027

Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays. / Ayala, Stuart Torres; Simbaña Vivanco, Lesly; Walas, Nikolina et al.
En: Open Forum Infectious Diseases, Vol. 12, N.º 2, ofaf027, 01.02.2025.

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

TY - JOUR

T1 - Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador

T2 - Comparison of xTAG-GPP and TaqMan Array Card Assays

AU - Ayala, Stuart Torres

AU - Simbaña Vivanco, Lesly

AU - Walas, Nikolina

AU - Jesser, Kelsey

AU - Zhou, Nicolette A.

AU - Fagnant-Sperati, Christine S.

AU - Burroughs, Hadley R.

AU - Lee, Gwenyth O.

AU - Eisenberg, Joseph N.S.

AU - Trueba, Gabriel

AU - Levy, Karen

AU - Arnold, Benjamin F.

PY - 2025/2/1

Y1 - 2025/2/1

N2 - We compared the performance of 2 multiplex platforms, Luminex xTAG Gastrointestinal Pathogen Panel and TaqMan Array Card, against a panel of 14 enteric pathogen targets in a community-based birth cohort in Ecuador. We found high levels of agreement and similar prevalence estimates across most pathogens.

AB - We compared the performance of 2 multiplex platforms, Luminex xTAG Gastrointestinal Pathogen Panel and TaqMan Array Card, against a panel of 14 enteric pathogen targets in a community-based birth cohort in Ecuador. We found high levels of agreement and similar prevalence estimates across most pathogens.

KW - bacteria

KW - enteric pathogens

KW - multiplex PCR

KW - protozoans

KW - viruses

UR - https://www.scopus.com/pages/publications/85217258521

U2 - 10.1093/ofid/ofaf027

DO - 10.1093/ofid/ofaf027

M3 - Artículo

AN - SCOPUS:85217258521

SN - 2328-8957

VL - 12

JO - Open Forum Infectious Diseases

JF - Open Forum Infectious Diseases

IS - 2

M1 - ofaf027

ER -

Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays

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