Optimization of the extraction and precipitation process of a leaf protein concentrate from Moringa oleifera Lam.

Título traducido de la contribución: Optimización del proceso de extracción y precipitación de un concentrado proteico foliar de Moringa oleifera Lam.

Byron Pérez, Javier Garrido, Andrea Endara, Andrea C. Landázuri, Lucía Ramírez-Cárdenas

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

2 Citas (Scopus)

Resumen

This study aimed to determine the best extraction and precipitation conditions of Moringa oleifera Lam. leaf protein. The influence of pH (10, 11, 12) and the concentration of NaCl (0, 0.25, 0.5) for the protein extraction process were studied through a Completely Randomized Design (CRD) with factorial arrange 32. The combination of pH 11 and 12 with 0 M NaCl had the best yield (P<0.05). The treatment of pH 11 without NaCl followed a precipitation stage for its purification, and the effect of different levels of pH (4, 4.5, 5) and temperature (40, 60, 80 °C) were evaluated using a CRD with factorial arrange 22 and 6 central points. The temperature did not affect the yield of the process in a significant way and the amount of precipitate was maximized at pH 4 and 4.5. From 100 g of the dry leaf, 7.26±0.19 g of protein was isolated with a recovery of 26.93±0.22 g 100 g-1 from the total protein. Due to their astringency and bitterness, consuming large amounts of Moringa oleifera Lam leaves is not a solution; therefore, obtaining a leaf protein concentrate could be useful for diverse applications in nutritional supplements, and as raw material for functional products development.

Título traducido de la contribuciónOptimización del proceso de extracción y precipitación de un concentrado proteico foliar de Moringa oleifera Lam.
Idioma originalInglés
Páginas (desde-hasta)9813-9821
Número de páginas9
PublicaciónRevista Facultad Nacional de Agronomia Medellin
Volumen75
N.º1
DOI
EstadoPublicada - 1 ene. 2022

Huella

Profundice en los temas de investigación de 'Optimización del proceso de extracción y precipitación de un concentrado proteico foliar de Moringa oleifera Lam.'. En conjunto forman una huella única.

Citar esto