Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells

Francesca Giampieri, Massimiliano Gasparrini, Tamara Y. Forbes-Hernandez, Luca Mazzoni, Franco Capocasa, Silvia Sabbadini, Josè M. Alvarez-Suarez, Sadia Afrin, Carlo Rosati, Tiziana Pandolfini, Barbara Molesini, José F. Sánchez-Sevilla, Iraida Amaya, Bruno Mezzetti, Maurizio Battino

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

78 Citas (Scopus)

Resumen

Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.

Idioma originalInglés
Páginas (desde-hasta)581-592
Número de páginas12
PublicaciónJournal of Agricultural and Food Chemistry
Volumen66
N.º3
DOI
EstadoPublicada - 24 ene. 2018
Publicado de forma externa

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