TY - JOUR
T1 - Phytochemical composition and cytotoxic effects on liver hepatocellular carcinoma cells of different berries following a simulated in vitro gastrointestinal digestion
AU - Giampieri, Francesca
AU - Afrin, Sadia
AU - Stewart, Derek
AU - McDougall, Gordon J.
AU - Brennan, Rex
AU - Blyth, Lesley
AU - Gasparrini, Massimiliano
AU - Mazzoni, Luca
AU - Capocasa, Franco
AU - Alvarez-Suarez, Josè Miguel
AU - Bompadre, Stefano
AU - de Oliveira, Pedro Nogueira Brás
AU - Santos, Claudia N.
AU - Masias, Manuel
AU - Agudo, Pablo
AU - Crespo, Jorge
AU - Mezzetti, Bruno
AU - Forbes-Hernández, Tamara Y.
AU - Battino, Maurizio
N1 - Publisher Copyright:
© 2018 by the authors.
PY - 2018
Y1 - 2018
N2 - Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p < 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the “IN” samples compared to the “OUT” extracts, which represent the “serum-available” and the “colon-available” fractions, respectively. Incubation of the digested fraction for 24 h didn’t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion.
AB - Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p < 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the “IN” samples compared to the “OUT” extracts, which represent the “serum-available” and the “colon-available” fractions, respectively. Incubation of the digested fraction for 24 h didn’t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion.
KW - Berry
KW - Bioavailability
KW - Cytotoxicity
KW - In vitro gastrointestinal digestion
KW - Polyphenols
UR - http://www.scopus.com/inward/record.url?scp=85053274498&partnerID=8YFLogxK
U2 - 10.3390/molecules23081918
DO - 10.3390/molecules23081918
M3 - Artículo
C2 - 30071616
AN - SCOPUS:85053274498
SN - 1420-3049
VL - 23
JO - Molecules
JF - Molecules
IS - 8
M1 - 1918
ER -
