Population structure and genetic diversity of Mycobacterium tuberculosis in Ecuador

Daniel Garzon-Chavez, Miguel Angel Garcia-Bereguiain, Carlos Mora-Pinargote, Juan Carlos Granda-Pardo, Margarita Leon-Benitez, Greta Franco-Sotomayor, Gabriel Trueba, Jacobus H. de Waard

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

13 Citas (Scopus)

Resumen

Tuberculosis (TB) is a significant public health problem in Ecuador with an incidence of 43 per 100,000 inhabitants and an estimated multidrug-resistant-TB prevalence in all TB cases of 9%. Genotyping of Mycobacterium tuberculosis (MTBC) is important to understand regional transmission dynamics. This study aims to describe the main MTBC lineages and sublineages circulating in the country. A representative sample of 373 MTBC strains from 22 provinces of Ecuador, with data comprising geographic origin and drug susceptibility, were genotyped using 24 loci-MIRU-VNTR. For strains with an ambiguous sublineage designation, the lineage was confirmed by Regions of Difference analysis or by Whole Genome Sequencing. We show that lineage 4 is predominant in Ecuador (98.3% of the strains). Only 4 strains belong to lineages 2-sublineage Beijing and two strains to lineage 3-sublineage Delhi. Lineage 4 strains included sublineages LAM (45.7%), Haarlem (31.8%), S (13.1%), X (4.6%), Ghana (0.6%) and NEW (0.3%). The LAM sublineage showed the strongest association with antibiotic resistance. The X and S sublineages were found predominantly in the Coastal and the Andean regions respectively and the reason for the high prevalence of these strains in Ecuador should be addressed in future studies. Our database constitutes a tool for MIRU-VNTR pattern comparison of M. tuberculosis isolates for national and international epidemiologic studies and phylogenetic purposes.

Idioma originalInglés
Número de artículo6237
PublicaciónScientific Reports
Volumen10
N.º1
DOI
EstadoPublicada - 1 dic. 2020

Huella

Profundice en los temas de investigación de 'Population structure and genetic diversity of Mycobacterium tuberculosis in Ecuador'. En conjunto forman una huella única.

Citar esto