TY - JOUR
T1 - SARS-CoV-2 detection and sequencing in heart tissue associated with myocarditis and persistent arrhythmia
T2 - A case report
AU - Egas, Diego
AU - Guadalupe, Juan José
AU - Prado-Vivar, Belén
AU - Becerra-Wong, Mónica
AU - Márquez, Sully
AU - Castillo, Stalin
AU - Latta, Johanna
AU - Rodriguez, Francisco
AU - Escorza, Giovanni
AU - Trueba, Gabriel
AU - Grunauer, Michelle
AU - Barragán, Verónica
AU - Rojas-Silva, Patricio
AU - Cárdenas, Paúl
N1 - Publisher Copyright:
© 2021 The Author(s)
PY - 2021/1
Y1 - 2021/1
N2 - Background: SARS-CoV-2 uses the human cell receptor angiotensin-converting enzyme (ACE2). ACE2 is widely present in the cardiovascular system including the myocardium and the conduction system. COVID-19 patients that present severe symptoms have been reported to have complications involving myocardial injuries caused by the virus. Here we report the detection of SARS-CoV-2 by whole genome sequencing in the endocardium of a patient with severe bradycardia. Case presentation: We report a case of a 34-year-old male patient with COVID-19 tested by PCR, he started with gastrointestinal symptoms, however, he quickly deteriorated his hemodynamic state by means of myocarditis and bradycardia. After performing an endocardium biopsy, it was possible to identify the presence of SARS-CoV-2 in the heart tissue and to sequence its whole genome using the ARTIC-Network protocol and a modified tissue RNA extraction method. The patient's outcome was improved after a permanent pacemaker was implanted. Conclusions: It was possible to identify a SARS-CoV-2 clade 20A in the endocardium of the reported patient.
AB - Background: SARS-CoV-2 uses the human cell receptor angiotensin-converting enzyme (ACE2). ACE2 is widely present in the cardiovascular system including the myocardium and the conduction system. COVID-19 patients that present severe symptoms have been reported to have complications involving myocardial injuries caused by the virus. Here we report the detection of SARS-CoV-2 by whole genome sequencing in the endocardium of a patient with severe bradycardia. Case presentation: We report a case of a 34-year-old male patient with COVID-19 tested by PCR, he started with gastrointestinal symptoms, however, he quickly deteriorated his hemodynamic state by means of myocarditis and bradycardia. After performing an endocardium biopsy, it was possible to identify the presence of SARS-CoV-2 in the heart tissue and to sequence its whole genome using the ARTIC-Network protocol and a modified tissue RNA extraction method. The patient's outcome was improved after a permanent pacemaker was implanted. Conclusions: It was possible to identify a SARS-CoV-2 clade 20A in the endocardium of the reported patient.
KW - Arrhythmia
KW - COVID-19
KW - MinION
KW - Myocarditis
KW - Pacemaker
KW - SARS-CoV-2
UR - http://www.scopus.com/inward/record.url?scp=85107957164&partnerID=8YFLogxK
U2 - 10.1016/j.idcr.2021.e01187
DO - 10.1016/j.idcr.2021.e01187
M3 - Artículo
AN - SCOPUS:85107957164
SN - 2214-2509
VL - 25
JO - IDCases
JF - IDCases
M1 - e01187
ER -
