Trypanosoma cruzi isoenzyme variability in Ecuador: First observation of zymodeme III genotypes in chronic chagasic patients

E. A. Garzón, C. Barnabé, X. Córdova, C. Bowen, W. Paredes, E. Gómez, A. Ouaissi, Michel Tibayrenc, A. G. Guevara

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

20 Citas (Scopus)

Resumen

We have analysed by multilocus enzyme electrophoresis (MLEE) at 21 genetic loci 10 Trypanosoma cruzi stocks isolated from chronic chagasic patients and 3 stocks isolated from Triatoma dimidiata collected in human habitats from the coastal part of Ecuador (all stocks isolated in August-December 1998). Isoenzyme profiles were compared to those of 4 laboratory-cloned stocks representing the major phylogenetic subdivisions of T. cruzi. This parasite's genetic variability in Ecuador proved to be considerable, even in this limited sample, since all main isoenzyme genotypes were recorded. Four stocks from patients were identical at all loci to the reference stock MNcl2 ('major clonet #39'; T. cruzi II) isolated in Chile. The 3 stocks isolated from T. dimidiata were closely related to the formerly described zymodeme I (T. cruzi I). Finally, 3 stocks from chronic chagasic patients (one with an asymptomatic form, 2 with a cardiac-digestive form) were closely related to the formerly described zymodeme III (presently not classified in either T. cruzi I or T. cruzi II). This is the first observation of this category of T. cruzi genotypes in chronic chagasic patients. In the past it was recorded only in acute patients, wild mammals and wild triatomine bugs. The epidemiological implications of these results are discussed.

Idioma originalInglés
Páginas (desde-hasta)378-382
Número de páginas5
PublicaciónTransactions of the Royal Society of Tropical Medicine and Hygiene
Volumen96
N.º4
DOI
EstadoPublicada - 2002
Publicado de forma externa

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